MmuINT0120845 @ mm9
Intron Retention
Gene
ENSMUSG00000026785 | Pkn3
Description
protein kinase N3 [Source:MGI Symbol;Acc:MGI:2388285]
Coordinates
chr2:29945359-29945695:+
Coord C1 exon
chr2:29945359-29945501
Coord A exon
chr2:29945502-29945587
Coord C2 exon
chr2:29945588-29945695
Length
86 bp
Sequences
Splice sites
5' ss Seq
GAGGTAAGT
5' ss Score
11.08
3' ss Seq
CTGAACCCCAATTTCCACAGTGT
3' ss Score
5.84
Exon sequences
Seq C1 exon
GAATTGGCTTTGGTGACCGGACCAGCACGTTCTGTGGCACCCCGGAGTTCCTGGCCCCCGAGGTTTTGACTCAGGAGGCTTACACGCGTGCTGTGGACTGGTGGGGGCTGGGTGTGCTGCTCTATGAGATGTTAGTGGGTGAG
Seq A exon
GTAAGTGCTAGAGCGGGGCTTCCAGGGTCTCTGGGGTGGGGACAGTGGGATAGCCTGCATCGCCTGCTGAACCCCAATTTCCACAG
Seq C2 exon
TGTCCATTCCCGGGGGACACAGAAGAGGAGGTATTTGAATGCATCGTCAGTGCTGATGTCCCATGTCCCCACTTTCTGTCAGTGCAAGGGCTCGAACTCATTCAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026785-Pkn3:NM_153805:19
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006920=Pkinase=FE(18.1=100)
A:
NA
C2:
PF0006920=Pkinase=FE(13.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGAGGTTTTGACTCAGGAGGC
R:
GCCCTTGCACTGACAGAAAGT
Band lengths:
176-262
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: