MmuINT0120851 @ mm9
Intron Retention
Gene
ENSMUSG00000026785 | Pkn3
Description
protein kinase N3 [Source:MGI Symbol;Acc:MGI:2388285]
Coordinates
chr2:29936276-29936756:+
Coord C1 exon
chr2:29936276-29936403
Coord A exon
chr2:29936404-29936572
Coord C2 exon
chr2:29936573-29936756
Length
169 bp
Sequences
Splice sites
5' ss Seq
GAGGTCAGG
5' ss Score
4.94
3' ss Seq
TGTTTGTGTCTCCTTGGTAGGCC
3' ss Score
8.14
Exon sequences
Seq C1 exon
GCCCCGACTTGCTGGCAGAGGAGCTGCAGCACCGACTTCGAGTAGAGGCTGCTGTGGCTGCAGGTGCCAAGAATGTGGTGAAGCTGCTTGGTGGCCAGAGGATGCAGGACCGAAAGGCACTGGCCGAG
Seq A exon
GTCAGGCTGTGTCCCCGGCTCACTGCTGTCTCTACAGCTCTGTTTGGGTCTATCCACATGCAGTGGGGCCTGCACCCAGGGTTGCTGCCTGTGGAGGGTAGTAGTGAATACCCATGAGGTGTGGCCTGACCCCTTTAACCCATACCTGGTGTTTGTGTCTCCTTGGTAG
Seq C2 exon
GCCCAGGCCCAGCTCCAGGAATCCTCCCAGAAGCTGGACCTCCTACGGCTGGCCCTGGAATTGCTGCTGGAGAGGTTGCCTCCCACCCACTCTCTACGTAGCAGGGTGACCCAAGAACTTTGGATGGCAATGCTTGGGAACCCCCAACCTTTGGGGACACTTGTGAAACCTATTGCCTTGACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026785-Pkn3:NM_153805:5
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.256 A=NA C2=0.038
Domain overlap (PFAM):
C1:
PF0218511=HR1=PD(2.7=4.7),PF0218511=HR1=PU(54.2=90.7)
A:
NA
C2:
PF0218511=HR1=PD(43.1=50.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGCACCGACTTCGAGTAGAGG
R:
GTTCCCAAGCATTGCCATCCA
Band lengths:
242-411
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: