MmuINT0131347 @ mm10
Intron Retention
Gene
ENSMUSG00000026821 | Ralgds
Description
ral guanine nucleotide dissociation stimulator [Source:MGI Symbol;Acc:MGI:107485]
Coordinates
chr2:28548955-28549532:+
Coord C1 exon
chr2:28548955-28549156
Coord A exon
chr2:28549157-28549289
Coord C2 exon
chr2:28549290-28549532
Length
133 bp
Sequences
Splice sites
5' ss Seq
AAGGTGACC
5' ss Score
6.78
3' ss Seq
GACACTGCTGTCGCCCACAGTTC
3' ss Score
9.08
Exon sequences
Seq C1 exon
CCGCCAGGCTCCCAGCACGGAGCTCAGCACCAGTAGCAGTGCCCACTCCAAGTCCTGTGACCAGCTTCGGTGCAGCCCTTACCTCGGCAGCGGGGACATCACCGACGCGCTCAGTGTGCACTCAGCTGGCTCTTCCAGCTCTGATGTGGAGGAGATCAACATGAGCTTCGTCCCAGAGTCTCCTGATGGCCAGGAAAAGAAG
Seq A exon
GTGACCACCCATGCTCCCTCCTCTACCCATCCTCACTTTGTCCAACAAGGGGCCCAGAAGTCCCCAAAGCTGGCTGCTGGCCTTCAGGGCCCTTCGTGGTTCAGGCTGCTGTTGACACTGCTGTCGCCCACAG
Seq C2 exon
TTCTGGGAGTCAGCCTCCCAGTCGTCCCCAGAGACCTCTGGCATCAGCTCCGCCTCCAGCAGCACCTCCTCTTCGTCAGCCTCCACCACGCCCGTGTCTACCACGCGCACCCACAAGCGCTCCGTCTCAGGGGTCTGCAGCTACAGCTCCTCACTGCCTCTCTACAACCAGCAGGTGGGCGACTGCTGCATCATCAGGGTCAGCCTGGATGTGGACAACGGCAACATGTACAAGAGCATCCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026821:ENSMUST00000028170:15
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.510 A=NA C2=0.443
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0078818=RA=PU(22.7=24.7)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCCAGCTCTGATGTGGAGGAG
R:
TGCTGGTTGTAGAGAGGCAGT
Band lengths:
242-375
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types