MmuINT0141117 @ mm10
Intron Retention
Gene
ENSMUSG00000030539 | Sema4b
Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B [Source:MGI Symbol;Acc:MGI:107559]
Coordinates
chr7:80220442-80220954:+
Coord C1 exon
chr7:80220442-80220557
Coord A exon
chr7:80220558-80220787
Coord C2 exon
chr7:80220788-80220954
Length
230 bp
Sequences
Splice sites
5' ss Seq
GGGGTGAGT
5' ss Score
7.93
3' ss Seq
CTTCATGTTTGGCTGTGTAGGGA
3' ss Score
5.52
Exon sequences
Seq C1 exon
GTGATGGCCGCCTGCACAAAGCAGTGACCCTGAGCTCCAGAGTCCACATCATTGAGGAGCTGCAGATCTTCCCTCAAGGACAGCCTGTGCAGAACCTGCTCTTGGACAGCCATGGG
Seq A exon
GTGAGTGGGCCATGGAGTGAATTTGACCACTTTGACTGTCAACAAGCCAGCTTTTCTGTCCCTTGCCAAAGCAAGATGGAAAGTTACAGAGCCCTGGCTTCTGACCATAAAAAGGATATGTGGCAGAACTTGTCAGGGGTGCCCTGGGATGACTAGTGAGCATGTGAGGCTAGTATGACAGTGTCCCGGTGCCTGTGTCTCCCTCTCACTCTTCATGTTTGGCTGTGTAG
Seq C2 exon
GGACTGTTGTATGCCTCCTCCCATTCCGGGGTGGTGCAAGTGCCCGTAGCCAACTGCAGCCTGTACCCAACCTGTGGAGACTGCCTCCTGGCTCGAGACCCCTACTGCGCCTGGACTGGCTCTGCCTGCAGGCTCGCTAGCCTCTACCAGCCTGATCTGGCCTCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000030539:ENSMUST00000032754:12
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(8.7=100)
A:
NA
C2:
PF0143720=PSI=PU(65.5=67.9)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATGGCCGCCTGCACAAAG
R:
GGCTGGTAGAGGCTAGCGAG
Band lengths:
266-496
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types