MmuINT0141175 @ mm10
Intron Retention
Gene
ENSMUSG00000025207 | Sema4g
Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G [Source:MGI Symbol;Acc:MGI:1347047]
Coordinates
chr19:44992138-44992882:+
Coord C1 exon
chr19:44992138-44992638
Coord A exon
chr19:44992639-44992733
Coord C2 exon
chr19:44992734-44992882
Length
95 bp
Sequences
Splice sites
5' ss Seq
AAGGTTAGG
5' ss Score
6.06
3' ss Seq
ACTGCCCCTCTCGTTTCTAGAGC
3' ss Score
10.55
Exon sequences
Seq C1 exon
AGAACAGACAGAGACCTGGAACTTGCCCTGAGACTGGGGGCAAGGTGACCCCTTCAGAACCCTACAGTCCTCCTGGGACCCTACACTGCTCACTCTCCATGGAATTTGCATTTGATACAGAAAGGCACGTGGTGACCCGCCCTTCCCTCTGACTTCTTACCTGTGATTCCATGGCCACACAACCCTGTGGCTCCATGTCCCCCCCATTCCATGATCCTCCATGGCCCCATGACTACTTGACCCCCATGACCTCATGACCCCTGACCCCCATGAGATCACACCCTGGACTTTGAACCCAGTGACTGCTCTTCCCCTCCCCTCCTGTGACTCTCCTGGGCTGCCCTCGGGGTCTTGTTGGTCTGAACTTCCGCAGGAAGATGTGGGGGAGGCTCTGGCCCCTCCTCTTCAGCTTTCTCACAGTAACAGCAGTGCCTGGACCCTCACTGCGGAGACCATCCCGAGAGCTAGATGCCACCCCACGGCTGACCATCTCCTATGAAG
Seq A exon
GTTAGGACCCCTCCCCCAACCTTGAAACACTATGGCTGCCAGAGCTGGGGACAGGAAGTAGTCCCCAGGTGGCTCACTGCCCCTCTCGTTTCTAG
Seq C2 exon
AGCTCTCCCAGATTCGGCACTTCAAGGGCCAAACCCAGAACTATTCAACACTGCTGCTGGAGGAGGCTTCTGAGAGGCTGCTGGTGGGGGCTCGAGGCGCCCTGTTCTCTCTCAGTGCCCGTGACATAAGAGATAGGACTCACAAAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000025207:ENSMUST00000026225:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.119 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0140314=Sema=PU(8.1=70.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGGGGTCTTGTTGGTCTGAAC
R:
TGAAGTGCCGAATCTGGGAGA
Band lengths:
181-276
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types