RnoEX0051744 @ rn6
Exon Skipping
Gene
ENSRNOG00000016047 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:RGD Symbol;Acc:1306057]
Coordinates
chr5:141157139-141166278:-
Coord C1 exon
chr5:141166143-141166278
Coord A exon
chr5:141164844-141164949
Coord C2 exon
chr5:141157139-141162457
Length
106 bp
Sequences
Splice sites
3' ss Seq
TTTTGCAAAAAAACAAACAGTCG
3' ss Score
0.02
5' ss Seq
TGGGTAAGT
5' ss Score
10.24
Exon sequences
Seq C1 exon
GCTTTCAGAAAGAGAGAAGGAACAGATCTCTGAGCAGTTGTGTGCCTTGAACAAGACTTACCATGATCTTTGTGATGGTTCTGCAAACCAACTCCAGCAGCTCCAGAGCGAGCTGGCTCAGCAGACAGAACAAAAG
Seq A exon
TCGCATGCTGGACGCTAACACCAAGCTTACACTGTGTATGTGATACGGCTGAGCTGCTCCGTAAGGCTCTATTTCCTATCTGCCTGAGGCGTGCTCTGCAGTGTGG
Seq C2 exon
GGTTGCAGAGCAGTTGCTGGGGTGATTGACCTAGGCACAGTGGAGATATTCCCCATCTTCAGAGCCATGCAAAAGGGCCTCATTGACCAAGACACAGGCCTTGTGCTCCTGGAATCCCAGGTTATCATGTCTGGCCTCATTGCTCCTGAGAACAGTGAAAAGCTCTCTTTGGAAGAGGGCCTCACCAGAAATCTCATTAGCCTTCCAATGTACCAACACCTCCTAGGACTAAGGGATTCTCTGTCTTTAATAAGTAGTCTTACAGGGACCCAAGGCTCCCTTTCTGTGGTGGAAGCAATTGAAAAGAAAATAGTCAGTGAGAGATTTGGACTGAAAGTCTTAGAAGTTCACCTGGCAACTGGAGGTTTCAGTCTCCCACCAAGTGAGAACTATATTAATCTGGAAGAAGCTTTTCATCAAGGCTTCATTGCATCATGGCTTCATTCAGAATTACAGTCTCACCTGAGAACATCCAAGAATTTGATAGACCCCAACACAGC
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000016047-'58-60,'58-59,59-60
Average complexity
C3
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.123 A=1.000 C2=0.384
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0068115=Plectin=WD(100=2.1),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.4),PF0068115=Plectin=WD(100=1.9),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.4),PF0068115=Plectin=WD(100=2.3),PF0068115=Plectin=WD(100=2.4),PF0068115=Plectin=WD(100=2.5),PF0068115=Plectin=WD(100=2.2)
Main Inclusion Isoform:
ENSRNOT00000084109fB8236
Other Inclusion Isoforms:
NA
Associated events
Conservation
Human
(hg19)
No conservation detected
Mouse
(mm9)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGCAGTTGTGTGCCTTGAACA
R:
GTTCTCAGGAGCAATGAGGCC
Band lengths:
257-363
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]