RnoINT0064442 @ rn6
Intron Retention
Gene
ENSRNOG00000061527 | Gck
Description
glucokinase [Source:RGD Symbol;Acc:2670]
Coordinates
chr14:86157752-86158992:-
Coord C1 exon
chr14:86158838-86158992
Coord A exon
chr14:86157872-86158837
Coord C2 exon
chr14:86157752-86157871
Length
966 bp
Sequences
Splice sites
5' ss Seq
ATGGTGAGC
5' ss Score
8.27
3' ss Seq
TGCCATGCTGCAACTCCTAGCTC
3' ss Score
3.43
Exon sequences
Seq C1 exon
AAGTCGGAGACTTTCTCTCCTTAGACCTGGGAGGAACCAACTTCAGAGTGATGCTGGTCAAAGTGGGAGAGGGGGAGGCAGGGCAGTGGAGCGTGAAGACAAAACACCAGATGTACTCCATCCCCGAGGACGCCATGACGGGCACTGCCGAGATG
Seq A exon
GTGAGCAGCCTGAGGGGCTGGCCAAGGAGGGCAAGGGGTCAAAGGTCACCCAGGATCTCGGGAGGGTTCCAAGAGCAGGAGTGATGGTGGACCACCCAGCTTCAGAGACCCATGAAGGTGGCTCGGAGGCTTAAAGTATTCCAAGTCCCAGTCCCCATCTGCCCAGAACAAGAAGGAGCTGGAGAAAGGTCAGTAGCAGGAGAAAAACCCAGCTTCTGTCCAGTTTCCTAGAGGAAAGCAAAGCATAGGATTGAGCAGCCAGAGGCCAGCTTTCCTCTCAGGCCACCATTACCCACCAGCAGGATAGACCAAGAGGCACGGTAGAAGCCACTGCCCAAGCAGCAAGGGTTTCTAAGCATGAGGAAGACTTAGATGGGAGACCTAGAAACAGAAGGAGCCTAGGATTAAGACAGACTTCCCTCACAGCCATCCCCGGGGGCAAGGCAAAACCCATTACCAGAGGGGCAGCCAGGTGAGAGGTTGCCCGTAGCCACTCAGCCTTCCCTTAGCACACCTTGAACTGGGCAGGCCATGACCCAGCACCCTCATCCATCTACAAAGAGCTCAGCAGTGCCCAGCCATGTGAGCAGCTCCACCGGAAGGGATTAGGACCAGCCTTCAGCGAGGGTCAGGTGGACAGGATACCTGGGGAACAGCAAAAGAGACAGGAATCATAGTCACGGCCAAGAATTCTACCCAGAGCTTCTTGGGACAGTCGTTTAGGCTTGAGGAGCCAGTCCTCCGCTGTGTCACCTGGTTCTGGGGACTGCAATCAGCAGGGTCTCCACCTAGGGAAGTGCAGGGCCTGAGTCCTGTTGAGAAGCAAACTCCAGACAGGTCCAGCCTACAGTTGCAGGCTCTGGGGTCAAATGTCATAACATCTCCGAGAGACACCGTGGCACCGTCCTTGGGAGAGAGAGCTGCTAGTGTCCAGGCCCTGACCTAATGCCATGCTGCAACTCCTAG
Seq C2 exon
CTCTTTGACTACATCTCTGAATGCATCTCTGACTTCCTTGACAAGCATCAGATGAAGCACAAGAAACTGCCCCTGGGCTTCACCTTCTCCTTCCCTGTGAGGCACGAAGACCTAGACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000061527:ENSRNOT00000080147:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.038 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0034916=Hexokinase_1=FE(44.7=100),PF0034916=Hexokinase_1=PU(2.4=3.8)
A:
NA
C2:
PF0034916=Hexokinase_1=PD(0.9=4.3),PF0034916=Hexokinase_1=FE(26.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTCTCCTTAGACCTGGGAGGA
R:
CTTGTCTAGGTCTTCGTGCCT
Band lengths:
262-1228
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]