RnoINT0084022 @ rn6
Intron Retention
Gene
ENSRNOG00000053691 | Lama5
Description
laminin subunit alpha 5 [Source:RGD Symbol;Acc:621023]
Coordinates
chr3:175567616-175568061:-
Coord C1 exon
chr3:175567917-175568061
Coord A exon
chr3:175567757-175567916
Coord C2 exon
chr3:175567616-175567756
Length
160 bp
Sequences
Splice sites
5' ss Seq
AAGGTGAGC
5' ss Score
9.6
3' ss Seq
TGATTGTCCCCTGCCCTTAGGAG
3' ss Score
9.82
Exon sequences
Seq C1 exon
ATGCAGACCCAATGTAGCTGGGCGTCGCTGTGACACCTGTGCACCAGGCTTCTATAGTTATCCTAGCTGTCGCCCCTGTGACTGTCATGAGGCAGGCACCATGGCCAGTGTGTGTGACCCCTTCACAGGCCAGTGCCATTGCAAG
Seq A exon
GTGAGCAGGGGCTTATGTCCAGGTCACCTTCACGGGCCCCTTCCTACAAATCTGCTTCAGCCGACCCATGGACTTCCTGGAAGCTTTTAGGGGGACAAGGCAAAAAAAACGGTCTAAGGTCTGGCTCTCAACACCAGCCTTGATTGTCCCCTGCCCTTAG
Seq C2 exon
GAGAATGTACAGGGCTCCAGATGTGACCAGTGTCGCGTGGGGACCTTCTCCTTGGATGCTGCTAACCCCAAGGGCTGTACCCGCTGCTTCTGTTTCGGAGCCACAGAGCGCTGTGGAAACTCTAACTATGCCCGCCATGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000053691:ENSRNOT00000081226:36
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0005319=Laminin_EGF=PD(51.0=51.0),PF0005319=Laminin_EGF=PU(44.9=44.9)
A:
NA
C2:
PF0005319=Laminin_EGF=PD(51.0=53.2)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGCAGACCCAATGTAGCTGG
R:
TCTGTGGCTCCGAAACAGAAG
Band lengths:
252-412
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]