RnoINT0099886 @ rn6
Intron Retention
Gene
ENSRNOG00000006783 | Neb
Description
nebulin [Source:RGD Symbol;Acc:1311134]
Coordinates
chr3:37747022-37748499:-
Coord C1 exon
chr3:37748395-37748499
Coord A exon
chr3:37747130-37748394
Coord C2 exon
chr3:37747022-37747129
Length
1265 bp
Sequences
Splice sites
5' ss Seq
GACGTGAGT
5' ss Score
9.22
3' ss Seq
ACTAAGGGCTCTTTCCACAGAAA
3' ss Score
6.11
Exon sequences
Seq C1 exon
CGCTTGTACACTGAAGCCTGGGACAACGACAAGAAAACCATTCATGTCATGCCGGACACACCAGAAATCATGCTGGCCAAACTCAACAGGATAAACTACAGCGAC
Seq A exon
GTGAGTGTCCAGAGAGGGGAAATGACGGGCCCTAATGCTTCCCTTAGTTAAATGCGTTAACTACTTTATTACTTATCCTATAAGTGACGTTGTTGGCAAAAGAAACAGTTCATCCTTGATTTAATCTTGAGCTATGTGGTGGGTCCACAAAGAAGCCCAGCCCTCAGTTATCAGTCAGGAAGTGGAGTGCTTATGGGGAAATAGTCTACCAGGGCCACTCCTCTCAAACCTTCTCTTGGGTCAGCCATGGGTACAAGGTCTATGTAGTCAATTGCTACAAGTGTGGTCCTGCATCTGTAGATTTGTCCCTGGTTTAAAGATACGCAAAAACACTTTAACTCTTCTGAAAGTGTATTGTGTTTTTTTATTGTTAACATTCCCTGAATCATATGTAACAACTTACATAGATTTATGTTGTATTATGTTTCATAAATAACTGGGAGATGATTTAAAAATATATAGGATGGTCAGGTAAGGTAGGTCATGTCTATAAATCCTAGCATGCAGAAGGAAAGCTGAGGCAGGAGGACCATGAGTTTGAGCATACTCTGGGCTACATAACAAGGTCTGGGAAGACCTGAGCTTTTTTAAGACTTGAAAATGGAGAGCTATACTGAGAGGTGTGCTTAGATGCCTACCCTCATTCATATAAGGGACTTGGATGTCAAAGGACTTCGTGTGGGACAAGAGCTCCTCGAACAAACCCTTTTCAGGCACTGAGGTAAAACCATTTTCAGTGAGTTACTATCCTCTTTCTCTGGTTTTCATTTGGTATTACTGCAGGTGTTTATCTGGACCAATATTTTTATAATGGCAATGACTCCAAATCAACCATGTTCCGCTATAGTAGCCCACATGCACTGGGTTTTCCAAAAAAAAAAAAAAAAAAAAAAAAACCAAATATATTCCATTTTAAAAACACTCTTCTTATTCCTTCTTATTTCATATCAAGAGAGCATTTTTAATATCCTGAGGTACTAGTAAGTGCTGGAATTTTTGTATGTGCTCCTTTGGGAAAATAAAATTTCTGCAACCAAATGACAATCTACTTCCAAACATTTTTTTTAGATTTCCCAGTCAGGCAAGCCTGACTTTTCATACACATGCCTGCGGATGTGTGGGCTGTGAAACATGCTGTGGTAGCTATGAAGCTACCAGAACATTTAGGTGATTCATTTCAGTCATTGAAATGAGGACAGAAAACAAAAATGAACAGACAGCAGCTTCATTTCTTCGGGAGTGGTGACTAAGGGCTCTTTCCACAG
Seq C2 exon
AAACTCTACAAACTGTCTCTGGAAGAGGCCAGGAGGCAAGGCTACGATTTGCGTGTGGACGCCATTCCCATCCGATCAGCCAAGGCATCAAGAGAGATTGCTAGTGAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000006783:ENSRNOT00000059461:32
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(57.1=45.7)
A:
NA
C2:
PF0088013=Nebulin=PD(35.7=27.8),PF0088013=Nebulin=PU(55.2=44.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTTGTACACTGAAGCCTGGG
R:
AGCAATCTCTCTTGATGCCTTGG
Band lengths:
206-1471
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]