RnoINT0099894 @ rn6
Intron Retention
Gene
ENSRNOG00000006783 | Neb
Description
nebulin [Source:RGD Symbol;Acc:1311134]
Coordinates
chr3:37800159-37800472:-
Coord C1 exon
chr3:37800368-37800472
Coord A exon
chr3:37800267-37800367
Coord C2 exon
chr3:37800159-37800266
Length
101 bp
Sequences
Splice sites
5' ss Seq
GACGTAAGC
5' ss Score
7.1
3' ss Seq
TTCTCTGGCTTCTGGTTTAGAAA
3' ss Score
8.29
Exon sequences
Seq C1 exon
AGGCTGTACACTGAGAAGTGGAACAAAGACAAGACCAGCATTCATGTCATGCCTGACACTCCAGACATCCTGCTCTCCAGAGTAAACCAGATCACCATGAGTGAC
Seq A exon
GTAAGCATATGAGCAGCAAAGAAGAGGGGGGATGCTGGAATCAGGGGCCTTGTTGTGATGGAAATAACAAGGCACTGTTGTTTCTCTGGCTTCTGGTTTAG
Seq C2 exon
AAACTATACAAGGCTGGCTGGGAAGAAGAGAAGAAGAAAGGCTATGATCTCAAGCCTGATGCCATCTCAATAAAGGCTGCAAAGGCCTCCAGAGACATTGCCAGTGAC
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000006783:ENSRNOT00000059461:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(55.2=45.7)
A:
NA
C2:
PF0088013=Nebulin=PD(37.9=30.6),PF0088013=Nebulin=PU(55.2=44.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGCTGTACACTGAGAAGTGGA
R:
CACTGGCAATGTCTCTGGAGG
Band lengths:
211-312
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]