RnoINT0142832 @ rn6
Intron Retention
Gene
ENSRNOG00000025711 | Spout1
Description
SPOUT domain containing methyltransferase 1 [Source:RGD Symbol;Acc:1563514]
Coordinates
chr3:8747795-8748604:-
Coord C1 exon
chr3:8748445-8748604
Coord A exon
chr3:8747885-8748444
Coord C2 exon
chr3:8747795-8747884
Length
560 bp
Sequences
Splice sites
5' ss Seq
CAAGTGAGT
5' ss Score
9.1
3' ss Seq
GCCTGTCTTAATCTTTCCAGGAG
3' ss Score
8.7
Exon sequences
Seq C1 exon
GACGGCCTTACACTCTGAGTGTGGCCCTGCCAGGCTCCATCCTGGACAACGCCCAGTCACCTGAACTCCGCACCTACCTGGCTGGCCAGATCGCCAGAGCCTGCGCCATCTTCTGTGTGGATGAGATTGTGGTGTTCGATGAGGAGGGCCAGGATACCAA
Seq A exon
GTGAGTCTTTGGGGAGAGGGGAGGACCAGCAGGGTGGCTGTGGCTCTGTCCTGCTCCTCGTGCCCTGCCCGGCTGCCTGGGAGGAGCTCTCAAAGGCAGCTGCAGATGAACCTTGTCTCTCCCTTGGTGCTAGAGAGTGGACTTGTTTTTGCCCATGCTCTACTCTGAAGCACATGACACTCAGCACTTCCCTGGTTGTGGGTTTGTGTAACATTTCTTTACGGTGCTTGTATACAAACACCAGTGTGGTTGTGGAGATTGGAATGCAGTTTGAGCTGAGCACTGGTGGCACACGCCTTTGATCCCAGTACCCAGGAGGCAGAGGCAGGCGGGTCTCTGAGAGTTCAGGGACAGCCTGGTCTACAGAGCAAGGAGGACTGCCAGGGCTACACCAAGAAACCAGTCTCCAAAAAGAAGTGGAAAAAAAAAAAAAACTGGTGGGAGTCAGATTGCCAGGCCTCCTTTCCCAATGGGGTTGTGGGCATGGAATTTCGGTCTTCAGGATTAGCCATCTCAGCAACCCAGCACTTGGGTCTGGAGGCCTGTCTTAATCTTTCCAG
Seq C2 exon
GAGTGTGGAGGGAGAATTCAGGGGCGTTGGCAAGAAGGGGCAGGCATGTGTCCAGCTGGCCCGCATCCTGCAGTACCTGGAGTGTCCACA
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000025711:ENSRNOT00000030623:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.056 A=NA C2=0.129
Domain overlap (PFAM):
C1:
PF0259812=Methyltrn_RNA_3=PU(16.8=90.7)
A:
NA
C2:
PF0259812=Methyltrn_RNA_3=FE(10.3=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GACGGCCTTACACTCTGAGT
R:
CCAGCTGGACACATGCCTG
Band lengths:
219-779
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]