BtaEX0002289 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000009696 | ACTN2
Description
Bos taurus actinin, alpha 2 (ACTN2), mRNA. [Source:RefSeq mRNA;Acc:NM_001034635]
Coordinates
chr28:9412605-9417857:+
Coord C1 exon
chr28:9412605-9412692
Coord A exon
chr28:9414231-9414309
Coord C2 exon
chr28:9417776-9417857
Length
79 bp
Sequences
Splice sites
3' ss Seq
CGACTGTGACTGTCGTGCAGCTG
3' ss Score
5.88
5' ss Seq
AAGGTCATT
5' ss Score
4.32
Exon sequences
Seq C1 exon
AAACCTCTGCCAAAGAAGGCCTGCTACTTTGGTGTCAAAGGAAGACTGCTCCTTATAGAAATGTGAACATTCAGAACTTCCATACCAG
Seq A exon
CTGGAAAGATGGCCTTGGACTCTGTGCGCTCATCCACAGACACCGGCCCGACCTCATTGACTACTCAAAGCTGAACAAG
Seq C2 exon
GATGACCCAATAGGAAATATCAACCTGGCCATGGAAATAGCAGAGAAGCACCTGGATATTCCCAAAATGTTGGATGCAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000009696_CASSETTE2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (disopred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0030726=CH=PU(24.0=83.3)
A:
PF0030726=CH=FE(25.0=100)
C2:
PF0030726=CH=FE(26.0=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCAAAGAAGGCCTGCTACTTTG
R:
CAACATTTTGGGAATATCCAGGTGC
Band lengths:
151-230
Functional annotations
There are 1 annotated functions for this event
PMID: 34816743
CRISPR/Cas9-mediated deletion of exon 6 from ACTN2 in hESCs, as well as forced expression of full-length ACTN2 in RBM24-/- hESCs, corroborated that inclusion of exon 6 is critical for sarcomere assembly.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development