GgaEX0021205 @ galGal3
Exon Skipping
Gene
ENSGALG00000014463 | ACTN2_CHICK
Description
NA
Coordinates
chr3:39143174-39148746:-
Coord C1 exon
chr3:39148659-39148746
Coord A exon
chr3:39146508-39146586
Coord C2 exon
chr3:39143174-39143255
Length
79 bp
Sequences
Splice sites
3' ss Seq
TTTTTTTTTCTGTCGTGAAGCTG
3' ss Score
6.24
5' ss Seq
AAGGTCAAC
5' ss Score
2.22
Exon sequences
Seq C1 exon
AGACCTCTGCCAAAGAAGGGCTGCTGCTGTGGTGTCAAAGAAAAACTGCTCCTTACAGAAATGTGAACATTCAGAACTTCCATCTTAG
Seq A exon
CTGGAAAGATGGCCTTGGATTATGTGCACTTATCCACAGACACCGACCTGACCTTATTGACTACTCCAAGCTAAATAAG
Seq C2 exon
GATGACCCCATAGGAAATATCAACCTTGCCATGGAAATTGCTGAGAAACATCTGGATATCCCTAAGATGTTGGATGCAGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000014463_CASSETTE1
Average complexity
S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence exclusion
Show structural model
Features
Disorder rate (disopred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0030726=CH=PU(24.0=83.3)
A:
PF0030726=CH=FE(25.0=100)
C2:
PF0030726=CH=FE(26.0=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGACCTCTGCCAAAGAAGGG
R:
TCTGCATCCAACATCTTAGGGA
Band lengths:
168-247
Functional annotations
There are 1 annotated functions for this event
PMID: 34816743
CRISPR/Cas9-mediated deletion of exon 6 from ACTN2 in hESCs, as well as forced expression of full-le... [Show more]