BtaEX0036258 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000006222 | TFDP2
Description
Bos taurus transcription factor Dp-2 (E2F dimerization partner 2) (TFDP2), mRNA. [Source:RefSeq mRNA;Acc:NM_001075241]
Coordinates
chr1:127982126-128002248:+
Coord C1 exon
chr1:127982126-127982247
Coord A exon
chr1:127985248-127985295
Coord C2 exon
chr1:128002086-128002248
Length
48 bp
Sequences
Splice sites
3' ss Seq
TTTTTTTTTTTTCTTTTCAGTGA
3' ss Score
11.66
5' ss Seq
AAGGTGAGT
5' ss Score
10.47
Exon sequences
Seq C1 exon
ATTATAAGCACACCGCAGAGACTAACCAGTTCAGGAAGTGTTCTGATTGGGAGTCCATACACCCCTGCTCCAGCAATGGTTACTCAGACACACATAGCAGAAGCTGCTGGCTGGGTCCCAGG
Seq A exon
TGATAGAAAACGGGCTAGAGAATTTATAGACTCTGATTTTTCAGAAAG
Seq C2 exon
CAAACGAAGCAAAAAAGGAGATAAAAATGGAAAAGGCTTGAGACATTTTTCAATGAAAGTGTGTGAGAAAGTTCAGCGGAAAGGCACAACGTCATATAATGAAGTAGCTGATGAGCTGGTGTCCGAGTTCACCAATTCAAACAACCACTTGGCAGCTGATTCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000006222_CASSETTE2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.333 A=0.529 C2=0.382
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0231915=E2F_TDP=PU(56.1=83.6)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTCCAGCAATGGTTACTCAGA
R:
TGTGCCTTTCCGCTGAACTTT
Band lengths:
144-192
Functional annotations
There are 2 annotated functions for this event
PMID: 8923205
Nuclear location when included and cytosolic when skipped. This forms heterodimer with E2F1 and translocates it to the nucleus.
PMID: 31771184
Overexpression of two splice isoforms of E2F dimerization partner 2 (Tfdp2) isoforms, splicing of which is regulated by Nova2, in HeLa cells show that the presence/absence of cassette exon HsaEX0064639 affects subcellular localization (absence of exon shifts the localization from exclusively nuclear to both nuclues and cytosol), interaction with E2F1 (absence of exon reduces interaction), and expression of Dll4 mRNA (E2F1 has known binding sites in the Dll4 promoter), with the isoform containing the exon leading to higher expression of Dll4 mRNA than the isoform without it.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development