MmuEX0047063 @ mm9
Exon Skipping
Gene
ENSMUSG00000032411 | Tfdp2
Description
transcription factor Dp 2 [Source:MGI Symbol;Acc:MGI:107167]
Coordinates
chr9:96188020-96195546:+
Coord C1 exon
chr9:96188020-96188141
Coord A exon
chr9:96190999-96191046
Coord C2 exon
chr9:96195384-96195546
Length
48 bp
Sequences
Splice sites
3' ss Seq
CTATCTTTTTGTCTTTTTAGTGA
3' ss Score
9.93
5' ss Seq
AAGGTGAGT
5' ss Score
10.47
Exon sequences
Seq C1 exon
ATTATAAGCACACCGCAGAGAATTGCCAATTCAGGAAGTGTTCTGATTGGGAATCCATATACCCCTGCACCCGCAATGGTCACTCAGACTCACATAGCTGAGGCTGCTGGCTGGGTTCCCAG
Seq A exon
TGATAGAAAACGAGCTAGAGAATTTATAGACTCTGATTTTTCAGAAAG
Seq C2 exon
TAAACGAAGCAAAAAAGGAGATAAAAATGGGAAAGGCTTGAGACATTTTTCAATGAAGGTGTGTGAGAAAGTTCAGCGGAAAGGCACAACGTCATACAATGAGGTAGCTGATGAGCTGGTATCTGAGTTTACCAACTCAAATAACCATCTGGCAGCTGATTCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000032411_MULTIEX1-7/7=6-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
Show structural model
Features
Disorder rate (Iupred):
C1=0.233 A=0.522 C2=0.336
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0231915=E2F_TDP=PU(56.1=83.6)
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCGCAATGGTCACTCAGACTC
R:
TGTGCCTTTCCGCTGAACTTT
Band lengths:
140-188
Functional annotations
There are 2 annotated functions for this event
PMID: 8923205
Nuclear location when included and cytosolic when skipped. This forms heterodimer with E2F1 and translocates it to the nucleus.
PMID: 31771184
Overexpression of two splice isoforms of E2F dimerization partner 2 (Tfdp2) isoforms, splicing of which is regulated by Nova2, in HeLa cells show that the presence/absence of cassette exon HsaEX0064639 affects subcellular localization (absence of exon shifts the localization from exclusively nuclear to both nuclues and cytosol), interaction with E2F1 (absence of exon reduces interaction), and expression of Dll4 mRNA (E2F1 has known binding sites in the Dll4 promoter), with the isoform containing the exon leading to higher expression of Dll4 mRNA than the isoform without it.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types