BtaEX6028081 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000032288 | GCK
Description
Bos taurus glucokinase (hexokinase 4) (GCK), mRNA. [Source:RefSeq mRNA;Acc:NM_001102302]
Coordinates
chr4:77847630-77849686:+
Coord C1 exon
chr4:77847630-77847792
Coord A exon
chr4:77848338-77848492
Coord C2 exon
chr4:77849567-77849686
Length
155 bp
Sequences
Splice sites
3' ss Seq
ACTTCTGTCTGTGGCTGCAGAAG
3' ss Score
7.61
5' ss Seq
ATGGTGAGC
5' ss Score
8.27
Exon sequences
Seq C1 exon
GCGGAACAGATCCTGGCAGAGTTCCAGCTGCAGGAGGAGGACTTGAAGAAGGTGATGAGGCGGATGCAGAAGGAGATGGACCGAGGCCTGAGGCTGGAGACCCACAAGGAGGCCAGTGTGAAGATGCTGCCCACCTATGTGCGCTCCACCCCAGAGGGCTCAG
Seq A exon
AAGTTGGGGACTTCCTCTCCCTGGACCTGGGTGGCACCAACTTCCGGGTGATGCTGGTGAAGGTGGGAGAAGGCGAGGCGGGACAGTGGAGTGTGAAGACCACGCACCAGATGTACTCCATCCCCGAGGATGCCATGACGGGTACTGCTGAGATG
Seq C2 exon
CTCTTTGACTACATCTCTGAGTGCATCTCTGACTTCCTGGATAAGCATCAGATGAAGCATAAGAAGCTGCCCTTGGGCTTCACCTTCTCCTTTCCCGTGAGGCATGAAGACATTGACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000032288-'1-3,'1-2,2-3=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.164 A=0.019 C2=0.000
Domain overlap (PFAM):
C1:
PF0034916=Hexokinase_1=FE(26.1=100)
A:
PF0034916=Hexokinase_1=FE(24.6=100)
C2:
PF0034916=Hexokinase_1=FE(18.8=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACAGATCCTGGCAGAGTTCCA
R:
CGGGAAAGGAGAAGGTGAAGC
Band lengths:
255-410
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]