DreEX6051111 @ danRer10
Exon Skipping
Gene
ENSDARG00000068006 | gck
Description
glucokinase (hexokinase 4) [Source:ZFIN;Acc:ZDB-GENE-060825-204]
Coordinates
chr8:40438160-40440857:+
Coord C1 exon
chr8:40438160-40438322
Coord A exon
chr8:40440256-40440410
Coord C2 exon
chr8:40440738-40440857
Length
155 bp
Sequences
Splice sites
3' ss Seq
ATTCATGTGACTCATCTCAGAGG
3' ss Score
3.91
5' ss Seq
ATGGTGAGA
5' ss Score
7.23
Exon sequences
Seq C1 exon
GTGGACCAAATTCTGTCTGAGTTTGTGTTGAGTAAAGAAGAGCTGGAGGAGGTGATGAGGCGAATGCGGAGAGAGATGGAGAGAGGACTGCGTGTGGAGACACATGATGAAGCCAGTGTCAAAATGTTGCCCACGTATGTCCGGTCCACACCTGAAGGCTCCG
Seq A exon
AGGTTGGTGATTTCCTGGCACTGGATCTTGGAGGGACAAATTTTCGAGTGATGCTTGTGAAAGTGGGTGAGGATGAAGAGCGAGGCTGGAAGGTGGAAACCAAACATCACATGTACTCCATCCCTGAAGATGCCATGACCGGCACAGCTGAAATG
Seq C2 exon
TTGTTTGATTACATTGCCGGCTGCATATCTGACTTCCTGGACAAACATAATCTCAAACACAAGAAGCTACCACTGGGATTCACCTTCTCTTTTCCAGTGCGTCACGAGGATTTGGACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000068006-'1-2,'1-1,2-2=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.164 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0034916=Hexokinase_1=FE(26.1=100)
A:
PF0034916=Hexokinase_1=FE(24.6=100)
C2:
PF0034916=Hexokinase_1=FE(18.8=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGAGCTGGAGGAGGTGATGAG
R:
TTGTCCAAATCCTCGTGACGC
Band lengths:
244-399
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]