BtaEX6077704 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000010057 | GZMB
Description
granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) [Source:HGNC Symbol;Acc:HGNC:4709]
Coordinates
chr21:35265818-35267979:-
Coord C1 exon
chr21:35267831-35267979
Coord A exon
chr21:35266440-35266587
Coord C2 exon
chr21:35265818-35265953
Length
148 bp
Sequences
Splice sites
3' ss Seq
GAACAGCCCTTTTCCTTCAGGGG
3' ss Score
6.2
5' ss Seq
AAGGTGAGG
5' ss Score
9.16
Exon sequences
Seq C1 exon
CTGACACTGTGTTTCTCTCCAAGAGGCTGGGGGATCTGAAAGAGAGCCAGGAGGTAGCACCAGCATCTCCATCCTGGGCAGTCTTTCTAGGAAGATGAAGCCTCTCCTGCTCCTGGTGGCCTTTCTCCTGACCCCCAGGGCAAAGGCAG
Seq A exon
GGGAGATCATCGGGGGCCATGAAGCCAAGCCCCACTCCCGCCCCTACATGGCATATCTTCAGTACTGGAATCAGGATGTCCAGAGTAGGTGCGGTGGGTTCCTGGTTCGACAGGACTTCGTGCTGACAGCCGCTCACTGCAACGGAAG
Seq C2 exon
CTCAATCAAAGTCACCCTGGGGGCCCACAACATCAAACAGCAGGAGAGGACCCAGCAGGTCATCAGGGTGAGAAGAGCCATCAGCCACCCTGACTATAATCCTAAGAACTTCTCCAACGACATCATGTTATTAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000010057-'0-2,'0-1,1-2=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.103 A=0.080 C2=0.043
Domain overlap (PFAM):
C1:
NO
A:
PF0008921=Trypsin=PU(21.5=94.0)
C2:
PF0008921=Trypsin=FE(20.5=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTTTCTCTCCAAGAGGCTGGG
R:
AGGATTATAGTCAGGGTGGCTGA
Band lengths:
242-390
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]