BtaINT0013174 @ bosTau6
Intron Retention
Gene
ENSBTAG00000006447 | ACSM3
Description
acyl-CoA synthetase medium-chain family member 3 [Source:HGNC Symbol;Acc:HGNC:10522]
Coordinates
chr25:18639618-18641552:+
Coord C1 exon
chr25:18639618-18639697
Coord A exon
chr25:18639698-18641428
Coord C2 exon
chr25:18641429-18641552
Length
1731 bp
Sequences
Splice sites
5' ss Seq
CAGGTAAGT
5' ss Score
10.86
3' ss Seq
CTTATCTGTTTTCTGAGAAGCTA
3' ss Score
3.44
Exon sequences
Seq C1 exon
ACACTTTCCAAGTTTCCCATCACAGTCTTCTGTTCAGCACCAACTGCATACAGAATGCTTGTACAGAATGATAAGGCCAG
Seq A exon
GTAAGTAAAGTTGGTAAATGGGCACTTATGGGCAGAGAGGAAAGGAAGAGGAAGATACAAAATAAAGGATCTCTCTGCCTAAAAACATATCTCCAGATCAGTAGGTATGGTATTTAGCAATCTTTTCTCTTCCTTGTCCTTCTTGCTTATATTCTGTACATGATAACTACTATATCCAACGTGGGTTCCCTCAAGCAGAGAGAGGAAAGATGGTTCAAGCCACATGTATAGCTCTGAGCCCAGTGTTTATCCTTTGTCTGTTACAAGAGCTGAGAAAATATACATCACCCTTATTCAAAACCCTATCCCAGCAAACAGGAAAGAAAATGAATGTCTTTTACTTTATTACTGATTCCATTTGAAAGTATTACATGGTCACTACTTTTAAACTGATTTAATTTTTAAATAGGAAACAATTCACATGACATAAAATTCAATAGGTATGTAAGAATATATAGTGAAAAGTCTTCCTCCACACCTTGTCTTCAGTTTTAGTTCTCCTTCTCCCTGAGATTTATGCCTGCACAAGCCAATATGCATATGTTCTCTCTCCACTCTTTTAAAATGAGACATGATATGCACGCAGTTCTGCCCCTTCTTTTCTTCACGTACAATACATTCTGAAACTGTGCTGTCCAATTTGGTAACCACTAGGAGAAGGCAATGGCACCCCACTCCAGTACTCCTGCCAGGAAAATCCCATGGACAGAGGGGCCTGGTGGGCTGCAGTCCATGGGGCCGCTAAGAATCGGACATGACTGGGTGACTTCACTTCCACTTTTATGCACTGGAGAAGGAAATGGCAACCCACTCCAGTGTTCTTGCCCGGAGAATCCCAGGGACGGGGCAGCCTGGTGGGCTGTCGTCTATGGGGTCGCACAGAGTTGAACACGACTGAAGTGACTTAGCAGCAGCAGCAGCAGCCACATATAGTTATTTACATTTTAATTAAATTAAAATTCAGCTCCAAACTATAATTAATAATACATTATTACATATTCAAAGTATTACATATTCGAAGTTGCTAAGACAGTAGATCTTAAAACTTCTCATCACAGGAAAAAATAGTCTTGTAACTATGTAGGGTAACAGATGTTAACTAGACTTGTTGTGCAATCATTTCACCATATGTACAAATATCAAATCACAACATTGTAAATCAAAAAGTAATATAACATTATAAGTCAATTATATTTCAATGAAAAAAAATTCAGTGCCTTGGTTACACTAGTCTTGTTTCAAGTGCCCAATAGTCACACAGGACTAGAGGATACAATAGTGGACAGCTCAGTATAGAAATTTCTGTCATAACAAAAAATTCTATTGGATAGTACAACCCAGGAGCATCTTCATTCCTTCTTTTTTTTTTTAACAACTGTATAGTGTTCTATTGTATAAATGCTCCACAATCAATTTAACCAATTAGAAAGCTTTTTATCACTGATTTAACATAATATTATGTGGTACCTTCCCTAGGGATGGCATGGTTTTTCTTTGCAAAAAGTCACGCCACCCCCAATCCTCCCCCTCCCTCCGCCCCCCACCAATAAAATGGAGAAAAAGAAAAAACAAACTTGGTGTTTCATGATAAAATTGTTTATTTTGTATCAGAGAGGAAAGTTAAGGGAAAAACTAAAACATTTTGTACTGAAATTTCACTAGAAATAATCCTAATTTCCATTTTTATCCATTTTCCTCCTAAATTTAACTCCTTATCTGTTTTCTGAGAAG
Seq C2 exon
CTATAAGTTCAAGAGCTTGAAGCACTGTGTAAGTGCTGGGGAACCAATCAACCCTGAAGTGACTGAACAATGGAGAAACAGGACAGGTCTGGATATCTACGAAGGATATGGACAGACTGAAACG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000006447:ENSBTAT00000008455:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.024
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(6.3=100)
A:
NA
C2:
PF0050123=AMP-binding=FE(9.9=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACACTTTCCAAGTTTCCCATCACA
R:
TCAGTCTGTCCATATCCTTCGT
Band lengths:
200-1931
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]