BtaINT0026746 @ bosTau6
Intron Retention
Gene
ENSBTAG00000002495 | BRD4
Description
bromodomain containing 4 [Source:HGNC Symbol;Acc:HGNC:13575]
Coordinates
chr7:8877668-8879272:+
Coord C1 exon
chr7:8877668-8877805
Coord A exon
chr7:8877806-8879136
Coord C2 exon
chr7:8879137-8879272
Length
1331 bp
Sequences
Splice sites
5' ss Seq
AAGGTGAGA
5' ss Score
8.68
3' ss Seq
GTGTGTGTCTCCTCTTTTAGCCT
3' ss Score
10.05
Exon sequences
Seq C1 exon
GATTACTATAAGATCATTAAAACACCTATGGATATGGGAACAATAAAGAAGCGCTTGGAAAACAACTATTACTGGAATGCTCAGGAATGTATCCAGGACTTCAACACTATGTTTACAAATTGTTACATCTACAACAAG
Seq A exon
GTGAGACGTGGGGGCTGGTGGAGAAGCAGAAGGTAGAGGAGGTGGTGGGCACGTCTGGAACCCATGGGCAGGGGTGCGGGGTGCAGGTAGAGTGGGGTCTGGCCAGAGGAGACCGCTGGCAGCAGAGGTAGGTGGGGCCGTTGGTTTTGCGTGTGAACTGGGTATGTGTCTTTTGAGGCCAGTAGCCTTCTGGCTGTGTTTCTCAGCCGTCCTCGTTAGCATGTGCTTCTGGAAGCATGTGCTTCTGATGCCCTTGGGACTTTGGTTCTTGAGCATGTCTAGTAGAGAAGATGTGAAAAGGACAGTAGTGAATCACTAAAAAGTTTGTTAGGAGACTCAAATTGTCAGGAAGGAATCTACTGAAATGTAGAGAAAATGTAAAGAAAGTGAAAGCTGCTTAGTTGTGTCCGACTCTTTTCGACCCCATGGGCTATACAGTCCATGAAATTGTCCAGGCCAATATACTGGCGTGGGTAGCCGTTCAGTTCTCCAGGGGATCTTCCCAACCCAGGGATTAAACAAAACATATCTCCTGCATTGCAGGCGGATTGTTTACCAGCTGAGCCACCAGGGAAGCCCAGTTGTAAAGAAAGATGTGCTCAGACAGAAACCTACATGTGAGAGAGGGTACAGTGTGGGGCCTGCTGCATTTGCAACTGTGGACATGACCATCAATAATAGCCTGTGGCCGGAGAATCAAGTATCCCCTTGTAGGGTCTTACCTGTAGGCCTAAAGATCACATCTTAACCCAATAGCCAAGCCATTCATCTTTGCTATGCCATGAAGCCAGATCATTTGTTTAAAGTCTCAGCTCTCATCGCTAGGTTTAAGGGAAATATGTACAGTTAGAGTTCTCCATTTAGAGCTGAGTGGGTTGAGGGATGTTACAGGTGAGGTGACAGAGTGGAACCCATGAGGCAGTAAGGAATGGACATTTCCCCAGGCCTCAGTGAGCTGCTTTGGCAACACCCTGGGGAACGTTATTGGAGCCCTGCTCATGTATCCTGTGCAATCCAAGTAATTCTGGTGAAGTTGCATCGTGTCAAGGGAGGGACCCCAGTCTGTGGTTGGCAGTGCTCGATAAATACTGCAGGCATTAGGATGATTTCTCAGGCCAGAAGTTAAAAGGCTGGAGAGGCAGCAGACCTGCAGGCCTAGGCAGCCTCTGCAGTGCCAGGTAGTATAGCTGTGTTGTGGCCCTTTGGAGTTCAGATTGGAGGACTCTGAATACGATGCTCCACACTTTGGGACCTAGGATGGGGAGCTCTTGTTCTGTCTAGAAAGGTGGGGAGGGCTTGCCAACCCTCACTGTGTGTGTCTCCTCTTTTAG
Seq C2 exon
CCTGGAGATGACATAGTCTTAATGGCAGAAGCTCTGGAGAAGCTCTTCTTGCAAAAAATCAACGAACTGCCCACTGAAGAAACTGAGATCGTGATAGTCCAGGCAAAAGGAAGAGGACGTGGGAGGAAAGAAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000002495:ENSBTAT00000003242:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.413
Domain overlap (PFAM):
C1:
PF0043920=Bromodomain=FE(52.3=100)
A:
NA
C2:
PF0043920=Bromodomain=PD(11.6=21.7)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATCATTAAAACACCTATGGATATGGG
R:
CTGCTTCTTTCCTCCCACGTC
Band lengths:
262-1593
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]