BtaINT0059577 @ bosTau6
Intron Retention
Gene
ENSBTAG00000032509 | FAM166B
Description
Bos taurus family with sequence similarity 166, member B (FAM166B), mRNA. [Source:RefSeq mRNA;Acc:NM_001038513]
Coordinates
chr8:60197223-60197681:-
Coord C1 exon
chr8:60197476-60197681
Coord A exon
chr8:60197275-60197475
Coord C2 exon
chr8:60197223-60197274
Length
201 bp
Sequences
Splice sites
5' ss Seq
CAAGTGAGA
5' ss Score
5.26
3' ss Seq
TATCAATTTATCCTCACAAGGCT
3' ss Score
3.35
Exon sequences
Seq C1 exon
GTTTTGTGCCACAGGCACAGTTCATCTTTGCCAAGAACTGCAGCCAGGTCTGGGCTGAGGCTCTGAATGGTTTTACTCAGAGGAACGGAGGACAGGGGAGTCAGGAGCTGCCCAAAGAGGCCAAGGGAGAAAAAGATGTGGAGAAAGACCAAGAGCCAAAGCCGGAGGTGGAAAAGGAGCCGGAGCTGGGGCAGGAGGCGGAACAA
Seq A exon
GTGAGATGAGAAGGCTGAGGCAGAGTCCAGTGGTGGGAGCGGGGAGCCCTTACCCCTGTGTGGCCAGGCCTGTGGTGGCCATGTGTAGTCTGGCTGTGGGCACAGGGCCAGACCACCACCTCCATTGCCCTGGGAGCTTGTGTTATAACCCAGGGGCTGAATTTGTTTGCCGTTTCTCCTCTATCAATTTATCCTCACAAG
Seq C2 exon
GCTTCCCCCTATTCCATGGATGACAGAGACCCTCGCAAGTTCTTCATGTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000032509:ENSBTAT00000046106:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.681 A=NA C2=0.444
Domain overlap (PFAM):
C1:
PF106294=DUF2475=PD(85.1=82.6)
A:
NA
C2:
PF106294=DUF2475=PU(8.8=27.8)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGCACAGTTCATCTTTGCCA
R:
GACATGAAGAACTTGCGAGGGT
Band lengths:
244-445
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]