BtaINT0070152 @ bosTau6
Intron Retention
Gene
ENSBTAG00000000704 | GUSB
Description
Bos taurus glucuronidase, beta (GUSB), mRNA. [Source:RefSeq mRNA;Acc:NM_001083436]
Coordinates
chr25:28170792-28171338:-
Coord C1 exon
chr25:28171186-28171338
Coord A exon
chr25:28170971-28171185
Coord C2 exon
chr25:28170792-28170970
Length
215 bp
Sequences
Splice sites
5' ss Seq
GACGTGAGT
5' ss Score
9.22
3' ss Seq
CTGTCTCTGCTGTCCTCCAGATC
3' ss Score
11.42
Exon sequences
Seq C1 exon
GTGAAGCTGACGGCGCAGACGGCCGTGGGGCCTGTGTCTGACTTCTACACCCTCCCGGTGGGGATCCGCACCGTGGCCGTCACCAAGAGCCAGTTCCTCATCAATGGGAAGCCATTCTATTTCCGAGGGGTCAACAAGCATGAGGATGCAGAC
Seq A exon
GTGAGTAGGGGCTCCTGGGTCCCTGTTTCTTGCCATCCCCTTCCCCATCCCCTGGAGTGGCTGAGGACAGCTCAGAACAAACTGCCCCAAACCACTGCCTATGCTTGGCCTGGATAGCAGGCTGATAGGGTGACTCCGGCGGGCCCAGCTCTTGGCAGAAACGTGTCTGGGATGGGGTCAAGTGCAGGGGCTCACCTGTCTCTGCTGTCCTCCAG
Seq C2 exon
ATCCGAGGGAAGGGCTTTGACTGGCCGCTGCTGGTGAAGGACTTCAACCTGCTTCGCTGGCTGGGCGCCAACGCTTTCCGCACCAGCCACTACCCCTATTCAGAGGAGGTGCTGCAGCTCTGTGACCGCTACGGGATCGTGGTCATCGACGAGAGCCCTGGTGTGGGCATCGTGCTGGT
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000000704:ENSBTAT00000000941:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0070316=Glyco_hydro_2=PD(21.6=43.1),PF0283612=Glyco_hydro_2_C=PU(8.6=51.0)
A:
NA
C2:
PF0283612=Glyco_hydro_2_C=FE(19.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGGGCCTGTGTCTGACTTCTA
R:
CACGATGCCCACACCAGG
Band lengths:
301-516
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]