MmuINT0075325 @ mm9
Intron Retention
Gene
ENSMUSG00000025534 | Gusb
Description
glucuronidase, beta [Source:MGI Symbol;Acc:MGI:95872]
Coordinates
chr5:130473887-130474423:-
Coord C1 exon
chr5:130474280-130474423
Coord A exon
chr5:130474066-130474279
Coord C2 exon
chr5:130473887-130474065
Length
214 bp
Sequences
Splice sites
5' ss Seq
GATGTGAGT
5' ss Score
7.77
3' ss Seq
GCAGCCCTGCTGTCTCCTAGATC
3' ss Score
5.83
Exon sequences
Seq C1 exon
GTGAAGGTGACAACAACTGAGTCTGTGACTGACTACTACACCCTTCCTATCGGGATTCGAACAGTGGCTGTCACAAAGAGCAAGTTCCTCATAAACGGGAAGCCCTTCTATTTCCAAGGGGTCAATAAGCACGAGGATTCAGAT
Seq A exon
GTGAGTAGGAGCTGTGGCATCAATGTGTGGCATGTTGGGAGTCATTGATATCTCCTTCCCCTACCTCTGTGCCCTGGGAAGATAGCATCGTGTGGTGCTGTTTGTGGTGAACCTACATAGGGTGGACGGGGTGAGTCCAGGGGCGGGGCTTTAGAAGAAAGTGCGAACTCTCCAGTCACAAGGGTAGAGGTCACGCAGCCCTGCTGTCTCCTAG
Seq C2 exon
ATCCGAGGGAAAGGCTTCGACTGGCCGCTGCTGGTAAAGGATTTCAACCTGCTCCGTTGGCTCGGGGCAAATTCCTTTCGTACCAGCCACTATCCCTACTCAGAGGAGGTACTTCAGCTCTGTGACCGATACGGGATTGTGGTCATCGATGAGTGTCCCGGTGTGGGCATTGTGCTACC
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000025534-Gusb:NM_010368:6
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0070316=Glyco_hydro_2=PD(19.2=39.6),PF0283612=Glyco_hydro_2_C=PU(8.6=54.2)
A:
NA
C2:
PF0283612=Glyco_hydro_2_C=FE(19.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGTGACAACAACTGAGTCTGTG
R:
GGGACACTCATCGATGACCAC
Band lengths:
299-513
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: