BtaINT0082800 @ bosTau6
Intron Retention
Gene
ENSBTAG00000009501 | KLKB1
Description
Bos taurus kallikrein B, plasma (Fletcher factor) 1 (KLKB1), mRNA. [Source:RefSeq mRNA;Acc:NM_001046352]
Coordinates
chr27:15326714-15328903:+
Coord C1 exon
chr27:15326714-15326772
Coord A exon
chr27:15326773-15328740
Coord C2 exon
chr27:15328741-15328903
Length
1968 bp
Sequences
Splice sites
5' ss Seq
GTGGTGAGT
5' ss Score
8.95
3' ss Seq
TTTCAATGATTCTGTCATAGGAT
3' ss Score
7.5
Exon sequences
Seq C1 exon
GATGATTGCACTCAGGCAAGCAGCTTACTTCATTTGCCTGTTTGCTACAGTTTCCTGTG
Seq A exon
GTGAGTGGATTATCTCTAAGATGCGAAGTTAAGGGTGAGGGGGAGAGTAATCAAGCAAGACATCCGACCCTCAGGACACATATCCTTTTAAGGTTCAGCACCAACTACCACTTGGCAGATATATCATCCTAGACACACCAGAAGGAGGGGCCCATAAACGAGAATGTCTTGGCAGGTGTCGACAGAAACTTCTGTTTGGAAAAGACTCTCCACAAAGCAGATCTTAAAACAGGCAGCCCTCTATTCTTATTATCAACATTAAAAAATCAACTTTATCTGAATATTCAGAAGAGTGCTAGGTATTTTATAGCATCTGGGTCCAACCCGAGTACCATAATATGTACTCAGTACATATTTTTACTCTTTTAGACTTCTGGAGAATCATTTCAGAAAAGGAAGTACCTAATTTATATTTAATTTAGGAAGTCATCTATTTGAATAGTTTGAGTTTCACCATCAGGTTTTTATCTTATTTCTCTTTCTATTCATTAAAATTTCACAGAATAGGAATGATTTTAGACACTGTTTTTCATTAATTATTGTAGAATGGAGTTTTTACGATGCCACCATTATGGGTGGTTTCTGAGTCTTTGTTGACTTAAGAAGACCACATCACAATGATGTTTATGGCTAAGACAAACACTTCTAAGTGTCTAGGGTGAGGGTTACATTTGGCAAAAAATAAATTTTTAAGGAAGAAGCTTTTATCCCCTAAACCAAACTCCCCTCCATCTGTCCCCTCATTCCAAATTGCTCTTCTGAGCCATAATCATTACGACATACAAATACACTGAATGCTGAAGAGTAGAAGCCAGAGATGCTGTGAACCATGGTCACTTCAATTTTATTATTTTTGAAAATAATTATGATCATGCTAGGGAAATTAGAGGTGCAAATTAGCAGAAAATGAGAAAATACCTGTCATCTGTAGTCTTATAATACAGAGATGGGCTTTGTTAAAAATATCTTTTTCAGATATTTTTCTGCATGTGTCTTTATACACTCTGTTATTAGATACTTTTTTGTAACTTATGTTTCCCATTTATAGTGATCTTTCAAACAGATAAATATATATCATGTTTTTGGAGCTTAATATTCTTCTCTATCAGAGAAGGCAATGGCAACCCACTCCAGTACTCTTGCCTGGAAAATCCCATGGATGGAGGAGCCTGGTAGGCTGCAGTCCATGGGGTTGTGAAGCGTCAGACACGACTGAGTGACTTCACTTTCACTTTTCACTTTCATGCATTGGAGAAGGAAATGGCAACCCATTCCAGTGTTCTTGCCTGGAGAATCCCAGGGACGGGGGAGCCTGGTGGGCTGCCGTCTATGGGGTCGCATAGAGTCGGACACGACTGAAGTGACTTAGCAGCAGCAGCATTCTTCTCTATTCATGATATTTTTGTATGCACATTCTTATATACATGTTTATTTCTGTAGAAAAAACTTTGGAAATTAAAACACCAGATATAGTAAAGTGTGCATATTTTTGAAGGTTTTATGATATATATCATCAAATTATCTTCCAGAAAAGTTGTTCCCATGTTAGAAGATAGGGAGAAACCAAGCTAAGATGGCATTTGAGGGAAAATAATTTATGTACAAATTATTATGGTTGAGCATTATAAATGAAAATCAACCAAGTATTTTTATGCATAGGTACCCAAAAACTAATTTTAACATCATGCATCAAGCCCTAATATAACACTATTTCAGGAATTCCTTGGCTGTCCAGTGGTTAGGACTCTGTGCTTCCACTACAGACATGGGTTCGAGCCCTGGTCAGGGAACTAAGATCCCACATATTGTACAGCAGGTATATTTCATCCTGCTTCTGCATTTAGTCATGTCATAAGATCTCTGGGTATTCCATGGACCAATTAGGAAGGTTTAGAACGCAGATACATTTCTTTTAAAATTATTATTTCTTCCACATTAATGTGTCTTTTTTCAATGATTCTGTCATAG
Seq C2 exon
GATGCTTGACTCAACTATATCACAACATCTTCTTCAGAGGTGGGGATGTATCAGCCATGTACACCCCAGATGCCCAATACTGTCAGCTGATGTGCACCTTCCACCCTAGGTGCTTGCTCTTTAGTTTTCTTCCTGAAAATTCAACCAGTGATGCAGACAAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000009501:ENSBTAT00000012503:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0002421=PAN_1=PU(1.2=5.0)
A:
NA
C2:
PF0002421=PAN_1=FE(62.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATGATTGCACTCAGGCAAGC
R:
CTTTTGTCTGCATCACTGGTTGA
Band lengths:
222-2190
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]