BtaINT0100655 @ bosTau6
Intron Retention
Gene
ENSBTAG00000006907 | NEB
Description
nebulin [Source:HGNC Symbol;Acc:HGNC:7720]
Coordinates
chr2:44550623-44552357:+
Coord C1 exon
chr2:44550623-44550664
Coord A exon
chr2:44550665-44552156
Coord C2 exon
chr2:44552157-44552357
Length
1492 bp
Sequences
Splice sites
5' ss Seq
GAGGTAAGA
5' ss Score
10.06
3' ss Seq
ACTTCATGCCAAATTTTCAGACA
3' ss Score
5.26
Exon sequences
Seq C1 exon
TATTACACAGAGGAGACAGTCTATGAAGAGGTGCCAGGGGAG
Seq A exon
GTAAGATCCAGCCCACATCTGAACAGGCTGCATGCTCAATAAAGCAGAAGGTTTTCTCCTTTCTCAGACTCCCAGGGCCACCAAGCTGGCAAAAGGGAGAATCTGGAGGTCACTCACCTATGTGCCCAGCAGCCAGCAGTATACATGTGAATGTTACGTGTTTTTACTATTCAATATATCCAAAATGGCTAGGGATGGTTCCATTACTTGGGTGATATTTTTTGGTGTAGGTGATGTTTATTAGAATAATGCGATTGGCTATCACCACTCTCTGCAACAAAGGTGGGGCCTTGGACTTGAGATTTGCGTGGGGACCAGCAGTTTCACTGTGTAGAGTCATAACTTCTTGGGCACCAACTCTGCTCAAAAGAAATGAAGCCTCCAAGCCTTTTTCCTTGATGATAACGATAAAATGCTACTTAGAGTTTGGCACATTAGGAATTCTGAGAAAGTCATTTCCATAAATATTCTAAACTGGGACTTTTTCTTTTTTCAAGCTGAAATGTCTCTCATGCTTAATTTTCTGCATGGAGCTGCTGCTTTTAAGCATGACACATGGTGATATGGATGCCTAACCCTGAAACCTGCATAAAAACTAACAACTGTAGATTTTGTAAACTGGTTAGTTTCTCACCATCAACCATACATCAGTAGTAAGGGGCGGAGAGCAATCTATTAGCAATGATCTGACACAAATCAGGTTGTGTGTCATAGATGTACAATTTCCTTATTATTTAGGTTTCTCTTTTTCCTAATTGATGCTGGAACTCTTCCTTTAGTACAGATGATATAAGCTGGGAAGGAAATTTTACTCTGAGATGCATTGAAAATTTAACTGCTTATCTACTTTTCTTACACATTTTTTAACTTTGGATTAGACCCAAACTTTGGACCTTGGTTTCTTCCACTATAAAGTCTATTGTAATAAGCAGCAGTTTCCCACCTACCACATACTGTTGTGAAGCTAACTACCCCATTATGTTGTCATCATCATTCATGTTCACGTAGGCATTTATTAGTGTAAATGGCACTCTAGTTCACTTTAATTAACTAAGCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATTGAGAATATTTTTGTTTTATTTTTATCCTATCTTCAAGTGTTCCTTCAGTCAAATGGAGTTTTAAGGCAATGTTGCAAGTAGGTAAGAAGTAACTTTTTTGTTCCAGGCAATCTGACACAGTCAGCATTCAGACTTATGTAACATAATGTGCCTTCATGTGGATATGGCATGTGATTTCCTGCTTTGGCCTGTTTTAGATGTGAAGTATTAATAACACCAGGAATATCGATCTGACTTGAAGTTAGAATAACGTATATCAAGATCATAGTATTACAGTTTTTACTTTCATAACATTGTCAAATTTTGATACAACTTCATGCCAAATTTTCAG
Seq C2 exon
ACAAGAACAAGATTCTATGAAACTACGACAACAAGGACATCTGACTATGAGCAATCAGAAACCTCCAGACCGGCCCTGGCACAGCCAGTACCTGAAAAGCCGGTGGAGAGGAAGAGGGTTATTCGAAAGAAGGTGGACCCTTCAAAGTTCATGACCCCCTACATTGCACACAGTCAGAAAATGCAGAATCTCTTTAGCACA
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000006907:ENSBTAT00000055345:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.714 A=NA C2=0.642
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0088013=Nebulin=PU(55.2=23.9)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACACAGAGGAGACAGTCTATGA
R:
TGTGCTAAAGAGATTCTGCATTTTCT
Band lengths:
239-1731
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]