BtaINT0103667 @ bosTau6
Intron Retention
Gene
ENSBTAG00000019327 | NRAP
Description
nebulin-related anchoring protein [Source:HGNC Symbol;Acc:HGNC:7988]
Coordinates
chr26:34340208-34341600:-
Coord C1 exon
chr26:34341289-34341600
Coord A exon
chr26:34340340-34341288
Coord C2 exon
chr26:34340208-34340339
Length
949 bp
Sequences
Splice sites
5' ss Seq
GATGTGAGC
5' ss Score
4.72
3' ss Seq
GCCTCCCTCGTTCCCCCAAGGTC
3' ss Score
8.69
Exon sequences
Seq C1 exon
TTCCGGTACAAAGAGGCCTTCCTACGGGACCGGGGCCTGCAGATAGGGTACCGCAGCATCAACGACGACCCGAGGACGAAGCATTTCCTCAGCGTCGGCAGACTCCAGAGTGACAACGAGTACAAGAAGGACTTCGCCAAGAGTCGGTCCCAGTTCCACAGCCGCCCAGACCAGCCCGGCTTCCTCCAGGCCAAGAGGAGCCAGCAGCTGGCCAGCGACGTGCACTACAGGCAGCCCCTGCCCCAGCCCACCTGCGACCCGGAGCAGCTGGGCCTGAAACACGCGCAGAAGGCCCACCGGCTGCAGAGCGAT
Seq A exon
GTGAGCTGCCCTCCCCTCAGGCCTAGGCCCTGCCTCTGGTTCTCTTCATCAGAGGTGTCCATAATCCCACGTCCCGTGTTACCCTCAGGGAGGAGCTGGGCTGCGATAGAACCTAGGAGACCTGAGATGATAGTGCCAGAAGAAATCACAAGAATCCCAGTGTATCGAGTCCTTCCTGTGGGCCAGGCTCCGTGCCAAATGCTTTCCATGCCGTGCCTCGTGGCTCTCACAGCCAGCTGGTTTACAGATGGGAACCGGAGGCTTAGAGCCATGAAGTCACTTACTCAGGATCCCATGGCAATCAGTAGCAGGTCTGGAATCTGAGCCCCATATGGCTTAATGTGAGAGCCCAAGGTATTGATTGTTGTGCCTTAGTCTTCCCAGGGCTTTCCAGGTGGCGCCAGTGGTAAAGAACCTGCTTGCCTGTACAGGAGATGTGAGAGACACAGGTTCGATCACTGGGTCAGGAAGATTCCCTGGAGAAGGGCATGGCAACCCACTCTAGTATTCTTGCCTGGGAAATCCCATGGACAGAGAAGCCTGATGGGCTACAGTCCGGCAAAGAGTCAGACATAACTGAAGCGACTTAGCACGTACACGCTCTTTCCAAGGGATCTTAGTAGTCAGCAGCCTGGGCCCTTTATCTCTGAGAGGCGACTGAAGCCCCTAAATGGGAAATGACTTCCTTAAGGTTACAGAGCAGGTCTAGATAGGAGCTCGGCCTTGAACCCTCACTGCAGCCCCCTTGAGTAGAGCAGTGCAGAGGACAGCGCTATGCTGGCCTGACCCCCAGCACAGCGCATCCGCAGTGCCAAGGAGCTCCTGCGTAAGGCGGCTTGGCTCCCACGTCCTGCTTTCACTAACAACATAGTCCCCAGGGCAGCCTTTCTAAGCCTCTCCACTCTAGGCCCTGCTGACCTATTCCTCTTGCCTCCCTCGTTCCCCCAAG
Seq C2 exon
GTCAAGTACAAATCAGACTTGAACCTGACCAGAGGTATTGGCTGGACACCTCCTGGCTCCTACAAAGTGGAAATGGCTCGGCGGGCTGCAGAATTGGCCAACGCGAGGGGCCTGGGTCTCCAAGGGGCTTAC
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000019327:ENSBTAT00000025740:40
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.654 A=NA C2=0.386
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=10.6),PF0088013=Nebulin=WD(100=27.9),PF0088013=Nebulin=WD(100=21.2),PF0088013=Nebulin=PU(53.6=14.4)
A:
NA
C2:
PF0088013=Nebulin=PD(39.3=25.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAAAGAGGCCTTCCTACGGGA
R:
TGTCCAGCCAATACCTCTGGT
Band lengths:
352-1301
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]