BtaINT0130849 @ bosTau6
Intron Retention
Gene
ENSBTAG00000001941 | SEMA4D
Description
Bos taurus sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (SEMA4D), mRNA. [Source:RefSeq mRNA;Acc:NM_001098160]
Coordinates
chr8:90236195-90237768:+
Coord C1 exon
chr8:90236195-90236346
Coord A exon
chr8:90236347-90237592
Coord C2 exon
chr8:90237593-90237768
Length
1246 bp
Sequences
Splice sites
5' ss Seq
AAGGTGAGC
5' ss Score
9.6
3' ss Seq
ACATGTATTTGGGGTTCCAGGGG
3' ss Score
5.44
Exon sequences
Seq C1 exon
AGCCCAGCTTCGTGTTTGCTGACGTGGTGCGAGAGAGCCCGGAGGGCGAGGACCTCGGGGACGACAGGGTGTACTTCTTCTTCACGGAGGTGTCTGTGGAGTACGAGTTTGTCTTCAAGCTGATGATCCCCCGGATAGCGAGGGTCTGCAAG
Seq A exon
GTGAGCCCACCCCCGCTTTTCTCTTTGGTCCCTGTACTTGGGGAGAGCACGTCACCTGGGCTCCACACTTCCTCTGAGGTGCCTGTCTCCTTCCTCATATTCATCCACACTACAGATAGTGGTGTGGATGGGCTGTTGGGCTCGGCCTTCATCCTTCCTACAGGTCGCCTTGGGGACCACAGGAGCACCAGCCCGTGTGTGACAGTGACTGGCTTTCTGTCCTCAGGGCTCTGTTCTTAACACATTCCTTAAATCTCTCCCAGTTTATAGGAAGTTAGTTTTCCTGTACTGTATTAACCAAAATCTATCCCTGGTGGCTCAGACGGTAAAGAATCCGCCTGGAATGCGGGAGACCCTGGTTCGGGAAGATCCCCTGGAGAAGGGAATGGCAGCCACACCAGGATTCTTGCCTGGAGAATTCCATGGACAGAGGTGTCTGAAAGGCTACAGTCCCTTGGATCACAAAGAGTTGGATGTGACTAAGCAACTATCACTTCCACTTTCACATTGTAATTAGGGGTTTTTCCACTCCAACATAAAGGTTGGGGAGTGCGTTTTCACTGTGTCCAGAGTGAGTGTGGACACTTTCAGTTTCATGATCTTCTGTGTCTAGTACTCAGAGTTTCAGTACTTCTGTCCAGACCCAGTCATGTTAGGAGTGTTCCATGATTGTTGCTTAGTGCTCCGTTCACTAAAGAGTGCTTTGGGGAACCTGATAGGTCCAGAGGATCTCAGCAGATAGCTTGAGAGCTGGAAGGAAGTAAAAATCTGATCTCCTTGAGGAATGCCTTTTAAGGGAGACCTTGTCTAAGCACACTGGCCAGAGACAGACTGGGGAGCCTGTGTGTCCACCAGCCCCTGGCTCTGCTGGTCGCTCACAGATGGGTCTTGAGTCTGGAGGTGCATGTGAGCCAAAGGGCTAAAGCGGTGACCGCGCAGGAGGACAGCAGGGCGTCTACTTCGTGACGCCGAGGGCGGGCGGAATCCAACAGTTGTTCTGTCTGCAGTTGGGTGACTGCTTGTTGAGGTGTTGTTGTGGGTTGGGTTTTGTGCTTTAATCCATGACCTGAGCTGGCACGGAAGGGGTTGCTGGGGTCACTGGTGGTCTGCAGCCGGCTTTCCTTTTTGGCCTCAGCTTCCTTTCACTGAGGCAGAGCTGCTCGGGGCTGGAGTACATCCCTCGTCACCGGCTCATGTTTGGGGGGAGCCTCCCCTCCTCTCACTGCACATGTATTTGGGGTTCCAG
Seq C2 exon
GGGGACCAGGGGGGCCTGAGGACCTTACAGAAGAAGTGGACCTCCTTCCTGAAGGCCAGGCTCATCTGCTCCCGGCCGGACAGCAACCTCGTCTTTAACGTGCTGCGGGATGTCTTCGTCCTCAGGTCTGCGGACCTGAAGGAACCCGTGTTCTACGGGGTCTTCACCCCGCAGCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000001941:ENSBTAT00000002525:9
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(11.5=100)
A:
NA
C2:
PF0140314=Sema=FE(13.4=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCCCAGCTTCGTGTTTGCT
R:
AGCTGCGGGGTGAAGACC
Band lengths:
327-1573
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]