BtaINT0165236 @ bosTau6
Intron Retention
Gene
ENSBTAG00000025124 | ZNF300
Description
zinc finger protein 300 [Source:HGNC Symbol;Acc:HGNC:13091]
Coordinates
chr7:64233853-64236648:-
Coord C1 exon
chr7:64236526-64236648
Coord A exon
chr7:64235394-64236525
Coord C2 exon
chr7:64233853-64235393
Length
1132 bp
Sequences
Splice sites
5' ss Seq
TAGGTAAGT
5' ss Score
9.66
3' ss Seq
TAATTTTCCTTTTTTTTTAGACA
3' ss Score
8.32
Exon sequences
Seq C1 exon
GGCATCCAGTTTCCAAACCAGATGTCATTTCCAAGTTGGAGCAAGGAGAAGATCCATGGATCATTAAAAGAGACATACCAAATTGGATCTGTCCATATGAAGGTCAGGCAGATGGGCGACTAG
Seq A exon
GTAAGTGAAATATTAGTTTGCTTGTTTTGTTTTTATTTTGGTTGATTGATGCCTGAGGCTTATGTTTCTCTCCTTAGTGAATTCTCTTACTCTTTGCTCCTGTACTGAAAATTTAGTTGGCACTCAACAAATAATTAATCAATTAAATAACTGTGAACCTTTATAGGGGATAGCTATTTCTTATTGATTCTATATTCTATTCTTATTGATTCTATTCTATATATTCTATCTTTTGTATTAAATGTATATTGTTTTATATATTTAATGCGTTTTATGAAAAGGCTTAATAGGCAATAGAAGGCAAATTTCTTCTAAAAACACAGGTTGCATTACAAAATTAAATGTCAAATACTGCTACTGTTTTTAAAAGTTCAGAGAAAATTAGAGTCTAAAGAAATACAACAGAATTTGAAAAGGCATTTTTCAGAGCATGCAGCTGTAGCTGCTTTGAAAGATAAATTGAGTATTGATGATGATGAGAAAGGGAGGTGAAGGCAAACTGTTGGTGAAGGTGGGCAAGAATATATGAACAAAACCTAGAAGATAGGGAAATACGGCTTTTCTGGGAGAACTTTGCTAGTTTTATTTGATAATATATAGAAGGAACAGTGGAATTCAAGCTTGGATTCTTGGCGTGACTCTGGTACTGAATCCACAGCATGATCTTGGGTTTTTCTCTTGTTTATATTAGTTGTTAATTAGGAAAGTAGAATAAGAGGAGATTAAATATTTTGAGTACAGAAGAGACCTTGTTTCTTCAGCTAGAGGTGGCAGGATAATACTAGCCAAGTATATAACAGATGGTTTATAAAATAAATGAATTTGAATGGTGAAAAGCATTTACATTCTGAAAGCAGGAGAAATGGGAAGATTCTAAAAGAGTAATCCAAGATTAGAGTGATAATAACTCAGATAGTTTTCTGTCTCCTTCAGGTCTAGTTTTTTCATGAATATTTCTCTTTAACATCTTTGTCTATTTATTACCCTGATCATAGTGTATGTTTTACTGTCTTCTGAAATGGCATCTCGTCCCTGTTTGGGTTCATCACCTTTATTAGATCAATCATTTTTTATACTCCTCTGTATTCTGTTAATTATTCTTTATGACTTTTTAATTTTCCTTTTTTTTTAG
Seq C2 exon
ACAGGAAGAGTAACCTTGACAACCCCCAATCATGTATTTTGGGGTCTGTTTCCTTCCATAATAAGATATTGAAAGGAGTCATAAAGGATGGTTCATTATACTCCATTTTAAAAGTCTGTCAAAGTGATGGCCAGTTACAGAGATGTCAGAAAAACAGACTTTCCAGACAAGTAACAGTCATCAACAACAAAACAATGACTGTAGAGTCAGACTACAGATATGATGCACTGAGGAAAATATTTAAAGAGTGCATAGAGTCAGATGCTTCAAGAAACAGACCCTATAACTATGATTCCTTTAAAAAGAACTCGAAATCTAATATTGACCTACCTAGTTGTAACAAGAACAATTCAAGAACAAACCTTGATGAAAGTTTTGAATGTGGAAAATCATTCATCCACAATGTGGCCAATTCTAACCTTGAGAAGCTTCACAATGGAGTAATTCCCTGTAATGATAATGAGCATGGAAATATTTTCAGCAAGAAACAATCCATTATTCTTTATCAGAATGTTGACACCAAGGAGAAAACCTGTGTGTGTATTACATGTGGAAAAGCCTTTGCTAAGAAGTCACAGCTCATTGTACATCAACGAATTCATACTGGGAAAAAACCATATGATTGTGGTGCATGTGGGAAAGCCTTCAGTGAGAAGTTTCACCTCATTGTACATCAGAGAACTCATACTGGGGAGAAACCTTATGAATGTTCTGAATGTGGAAAAGCCTTCTCTCAAAAATCATCCCTTATTATACATCAGAGAGTTCATACTGGAGAAAAACCATACGAATGTAGTGAATGTGGAAAAGCCTTCTCCCAGAAATCACCCCTCATTATACATCAGAGAATTCACACTGGAGAGAAACCTTATGAATGTAGAGAGTGTGGTAAGGCCTTCTCCCAGAAGTCACAACTGATTATACATCATAGAGCTCATACTGGAGAGAAGCCCTATGAGTGTACTGAATGTGGGAAAGCCTTCTGTGAGAAGTCCCACCTCATTATACATAAAAGAATTCATACTGGGGAGAAACCCTACAAATGTGCTCAGTGTGAGGAAGCCTTCAGCAGAAAGACAGAACTCATTACACACCAGTTAATTCATACTGGGGAGAAACCTTATGAATGTACTGCTTGTGGGAAGACCTTCTCCCGAAAGTCACAGCTCATTATACATCAGAGAACGCATACTGGAGAGAAACCCTATAAATGCAATGAATGTGGAAAAGCCTTCTGTCAGAAATCACATCTCATTGGACATCAGAGGATACACACAGGAGAAAAACCTTTTGTTTGTACTGAATGTGGGAAAGCTTTCTCTCAAAAGTCTCACCTCCCAGGTCATCAGAGGATTCATACAGGAGAGAAACCATATGTATGTGCTGAATGTGGAAAGGCGTTTTCTCAGAAGTCAGATCTTGTTTTACATCGGAGAATTCACACTGGGGAAAGACCCTATCGGTGTGCTGTATGTGGGAAAGCTTTCATCCAGAAATCACAACTCACTGTACATCAGAGAATTCATAGCAGTGGTAAAAAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000025124:ENSBTAT00000035145:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=0.071 A=NA C2=0.035
Domain overlap (PFAM):
C1:
PF0135222=KRAB=PD(0.1=0.0)
A:
NA
C2:
PF134651=zf-H2C2_2=WD(100=5.1),PF134651=zf-H2C2_2=WD(100=5.1),PF134651=zf-H2C2_2=WD(100=5.1),PF134651=zf-H2C2_2=WD(100=4.9),PF134651=zf-H2C2_2=WD(100=4.7),PF134651=zf-H2C2_2=WD(100=5.1),PF134651=zf-H2C2_2=WD(100=5.1),PF134651=zf-H2C2_2=WD(100=4.7),PF134651=zf-H2C2_2=WD(100=5.1),PF134651=zf-H2C2_2=WD(100=4.5),PF134651=zf-H2C2_2=WD(100=5.1)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Mouse
(mm10)
No conservation detected
Rat
(rn6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCAAGTTGGAGCAAGGAGAAGA
R:
GCATCTGACTCTATGCACTCT
Band lengths:
358-1490
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]