Special

DmeALTD0006095-4/13 @ dm6

Alternative 5'ss

Gene
Description
The gene Protein kinase N is referred to in FlyBase by the symbol DmelPkn (CG2049, FBgn0020621). It is a protein_coding_gene from Dmel. It has 17 annotated transcripts and 17 polypeptides (14 unique). Gene sequence location is 2R:9252310..9285097. Its molecular function is described by: protein serine/threonine kinase activity; protein kinase activity; ATP binding; protein binding; GTP-Rho binding. It is involved in the biological process described with 9 unique terms, many of which group under: cellular component organization; regulation of wound healing; localization; cell division; post-embryonic animal organ development. 65 alleles are reported. The phenotypes of these alleles manifest in: cellular anatomical entity; array of sensillum rows; non-connected developing system; adult head; intracellular non-membrane-bounded organelle. The phenotypic classes of alleles include: female semi-sterile; some die during larval stage; increased mortality; phenotype. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed at stages throughout embryogenesis, during late larval stages, at stages throughout the pupal period, in adult female stages.
Coordinates
chr2R:9283828-9285160:-
Coord C1 exon
chr2R:9285060-9285160
Coord A exon
chr2R:9284959-9285059
Coord C2 exon
chr2R:9283828-9284129
Length
101 bp
Sequences
Splice sites
5' ss Seq
GGAGTGCGT
5' ss Score
1.92
3' ss Seq
TGTTGCTTTTTCGGCCGCAGGAA
3' ss Score
10.74
Exon sequences
Seq C1 exon
CATACGAAAGTGTTCAATGGTATTTTCTTGGTATATTTAAAACCTATTCTGCATATTGTTCTCACTCTGGCGCGGTCACACTGGCTGTAACTCGAACATCC
Seq A exon
GTGTGTGTTGGGTTGTACTTTTGCGTTTTAAATTACAAAAACACAAATAAATACAGTGTTTATTTATTGGAAGCAGTCGGCGTTATGATTTGAAAATAGGA
Seq C2 exon
GAAATCAAATGAGAGCGCATTCGATAAAAAGAGATAAAGAAAATACTGTTCGGCATCAGGTGTTAAAGTGCTAAACAATTCCGAAAGCCAAAGGAAAGTAAACAAAAACGCGACGCGTGTGCTTGTGCTTGTGTGCGGCGCCAATAAAAGCCAAAAGCAAAATACTGACAACCAAAAAGATACACGCAACAACAAGAACCATATTCATAATAACCAACAACTAAATACTACAACAAGAAGCTACGGCATCGAGTGATTCAACGCGGCGCACCAAATAAACCATGTCGGATTCGTATTATCAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0020621-0-1,1-1,2-1,3-1,4-1,5-1,6-1,7-1,8-1,9-1,10-1,11-1,12-1-4/13
Average complexity
Alt5
Mappability confidence:
NA
Protein Impact

5' UTR

No structure available
Features
Disorder rate (Iupred):
  C1=NA A=NA C2=0.000
Domain overlap (PFAM):

C1:
NA
A:
NA
C2:
NO


Main Inclusion Isoform:
NA


Main Skipping Isoform:
NA


Other Inclusion Isoforms:
NA


Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TATTGTTCTCACTCTGGCGCG
R:
ATGGTTCTTGTTGTTGCGTGT
Band lengths:
250-351
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Neural diversity
  • Neurogenesis
  • Neuronal activity
  • Splicing factor regulation (brain)
  • Splicing factor regulation (SL2)