DmeEX0004468 @ dm6
Exon Skipping
Gene
FBgn0004516 | Gad1
Description
The gene Glutamic acid decarboxylase 1 is referred to in FlyBase by the symbol DmelGad1 (CG14994, FBgn0004516). It is a protein_coding_gene from Dmel. It has 5 annotated transcripts and 5 polypeptides (1 unique). Gene sequence location is 3L:4074077..4088744. Its molecular function is described by: glutamate decarboxylase activity; pyridoxal phosphate binding. It is involved in the biological process described with 8 unique terms, many of which group under: multicellular organismal process; cell junction organization; behavior; cellular component organization; response to abiotic stimulus. 39 alleles are reported. The phenotypes of these alleles manifest in: bouton; adult mushroom body anterior paired lateral cell; neuromuscular junction. The phenotypic classes of alleles include: phenotype; increased mortality during development; sensory perception defective; behavior defective. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of extremely low expression. Peak expression observed within 18-24 hour embryonic stages, during late pupal stages.
Coordinates
chr3L:4084730-4088744:-
Coord C1 exon
chr3L:4088212-4088744
Coord A exon
chr3L:4085429-4085529
Coord C2 exon
chr3L:4084730-4084798
Length
101 bp
Sequences
Splice sites
3' ss Seq
GTACTCGCACATTCCTCTAGCTA
3' ss Score
5.37
5' ss Seq
TCGGTAAGA
5' ss Score
10.53
Exon sequences
Seq C1 exon
ATTCGCTTGATTTGCCAAACCGAAAGGGGTTATGCAATCGCCTGTCTTTCGAACGCAACTGAGACTCCCACCTGGCATCCTGGCATTCTGGCATCCTGACAACGCTTTTGCAACGGGATTCTCGTCGAAAATACCTCACATAAATGCCTCAGCTAACCAACCTGCTGGAATCTTATTTTGTCAGCGTAATGACAGGCTAAACAGTCGAAATTCCCAAACTCCCATTTGTTTTCCACGGGCAACGAAAAATGTTCATGGCCCCCGGGGTGCGGAGCATTCCCTGGCCCACTGTGCTTCTGATGCCTCTGGCTGGCAGTTGAGAGTCGAGTGCCAAAGAGGACGCTTCGCCCTGGCCAGAAACGCCCGGTCTGAATTAGCCGCCCGAGAATAGCAGACCCAAGCTCCCAGCTCCATCCGCATCCGTGGGTGGCACTTGACTCCAAACATTTTCGCCTATCTGGGTTACAGGGCCTACTTGCGCTACGTGCAATCACAGCCAG
Seq A exon
CTATATCTTTTCGAAAGCCTCGTCGGTGATTCGTCGCCCCCCGCAGACAGTCAAAGTGAATTACCTAAAGCAGTATATCGACGATATCCGCAGGATTATCG
Seq C2 exon
AGGAGAAAATGTCGCTGAATCCCAACGGGTATAAACTGTCCGAGAGGACGGGCAAACTGACGGCATATG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0004516_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
5' UTR
No structure available
Features
Disorder rate (Iupred):
C1=NA A=NA C2=0.143
Domain overlap (PFAM):
C1:
NA
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGAGTCGAGTGCCAAAGAGGA
R:
ATATGCCGTCAGTTTGCCCG
Band lengths:
249-350
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)