DmeEX0005107 @ dm6
Exon Skipping
Gene
FBgn0264695 | Mhc
Description
The gene Myosin heavy chain is referred to in FlyBase by the symbol DmelMhc (CG17927, FBgn0264695). It is a protein_coding_gene from Dmel. It has 22 annotated transcripts and 22 polypeptides (20 unique). Gene sequence location is 2L:16766566..16788766. Its molecular function is described by 6 unique terms, many of which group under: nucleoside-triphosphatase activity; catalytic activity; hydrolase activity, acting on acid anhydrides; drug binding; actin filament binding. It is involved in the biological process described with 15 unique terms, many of which group under: epithelial cell development; adult somatic muscle development; regulation of biological quality; reproductive process; protein stabilization. 161 alleles are reported. The phenotypes of these alleles manifest in: somatic cell of ovariole; muscle attachment site; embryonic/larval heart; adult visceral muscle; germline cell. The phenotypic classes of alleles include: sterile; fertile; phenotype; increased mortality during development.
Coordinates
chr2L:16768584-16770837:+
Coord C1 exon
chr2L:16768584-16768884
Coord A exon
chr2L:16769459-16769602
Coord C2 exon
chr2L:16770457-16770837
Length
144 bp
Sequences
Splice sites
3' ss Seq
CGAACGAAAATGATAAACAGACA
3' ss Score
-2.01
5' ss Seq
TACGTAAGT
5' ss Score
9.27
Exon sequences
Seq C1 exon
GGAAGTTTTGGGCTCACGACGCGATTAACAAAGAAATTTTGAAAAGTCCCAATATTATTTTTCGAGTTTTTAAGTTTCTAAACGAAATTTAAGCAAGATGCCGAAGCCAGTCGCAAATCAGGAGGATGAGGATCCCACCCCATACCTGTTCGTGTCTTTGGAGCAAAGGCGTATCGATCAATCGAAACCCTATGACTCGAAGAAGTCTTGCTGGATCCCCGACGAGAAGGAGGGTTATCTCCTTGGTGAGATCAAGGCCACCAAGGGCGATATCGTCTCCGTTGGTCTGCAGGGTGGAGAG
Seq A exon
ACACGAGACTTAAAGAAAGATCTGCTCCAGCAAGTGAACCCCCCGAAATACGAAAAAGCCGAGGATATGTCCAACTTGACATACCTTAACGATGCCTCTGTGCTCCATAACTTGAGACAGAGATACTACAACAAGCTGATCTAC
Seq C2 exon
ACCTACTCCGGTCTTTTCTGCGTTGCCATCAATCCTTACAAGCGTTACCCCGTGTATACCAACCGTTGCGCTAAGATGTACCGTGGCAAGCGCCGTAATGAGGTGCCACCCCATATTTTCGCCATCTCTGACGGTGCCTACGTCGACATGTTGACCAACCACGTGAATCAATCTATGTTGATCACCGGTGAGTCTGGTGCCGGAAAGACTGAGAACACCAAGAAGGTCATTGCGTACTTCGCCACTGTTGGTGCCTCCAAGAAGACCGATGAGGCCGCCAAGAGCAAGGGCTCCCTGGAAGATCAGGTTGTGCAGACCAACCCTGTGCTTGAGGCCTTCGGTAACGCCAAGACCGTGCGTAACGATAACTCCTCTCGTTTC
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0264695-'10-6,'10-5,14-6
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.225 A=0.208 C2=0.086
Domain overlap (PFAM):
C1:
PF0273614=Myosin_N=PU(78.6=48.5)
A:
PF0273614=Myosin_N=PD(16.7=14.6),PF0006316=Myosin_head=PU(4.1=58.3)
C2:
PF0006316=Myosin_head=FE(18.6=100)
Main Inclusion Isoform:
FBpp0080453
Main Skipping Isoform:
FBpp0080452
Other Inclusion Isoforms:
FBpp0080454, FBpp0080457, FBpp0080458, FBpp0080459, FBpp0080460, FBpp0080462, FBpp0080463, FBpp0080464, FBpp0291041, FBpp0291043, FBpp0298830, FBpp0298831
Other Skipping Isoforms:
FBpp0080455, FBpp0080456, FBpp0291042, FBpp0298827, FBpp0298828, FBpp0298829, FBpp0312323
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal3)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGAGATCAAGGCCACCAAGG
R:
ACCAGACTCACCGGTGATCAA
Band lengths:
254-398
Functional annotations
There are 1 annotated functions for this event
PMID: 15345559
This event
Exchanging the flight muscle specific exon 3 region into the embryonic isoform (EMB-3b; DmeEX0005107) increased maximum power generation (P(max)) and optimal frequency of power generation (f(max)) threefold and twofold compared to fibers expressing EMB, whereas exchanging the embryonic exon 3 region into the flight muscle isoform (IFI-3a) decreased P(max) and f(max) to approximately 80% of IFI fiber values. Drosophila expressing IFI-3a exhibited a reduced wing beat frequency compared to flies expressing IFI, which optimized power generation from their kinetically slowed flight muscle. However, the slower wing beat frequency resulted in a substantial loss of aerodynamic power as manifest in decreased flight performance of IFI-3a compared to IFI. Thus the N-terminal region is important in tuning myosin kinetics to match muscle speed for optimal locomotory performance.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)