DmeEX0005310 @ dm6
Exon Skipping
Gene
FBgn0259111 | Ndae1
Description
The gene Na[+]-driven anion exchanger 1 is referred to in FlyBase by the symbol DmelNdae1 (CG42253, FBgn0259111). It is a protein_coding_gene from Dmel. It has 11 annotated transcripts and 11 polypeptides (10 unique). Gene sequence location is 2L:7223328..7249343. Its molecular function is described by: inorganic anion exchanger activity; anion:anion antiporter activity; sodium:bicarbonate symporter activity. It is involved in the biological process described with 6 unique terms, many of which group under: transport; establishment of localization; ion transmembrane transport; localization; inorganic ion transmembrane transport. 16 alleles are reported. The phenotype of these alleles manifest in: imaginal disc. The phenotypic classes of alleles include: viable; lethal. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 18-24 hour embryonic stages.
Coordinates
chr2L:7230889-7234997:-
Coord C1 exon
chr2L:7234527-7234997
Coord A exon
chr2L:7233681-7233806
Coord C2 exon
chr2L:7230889-7231183
Length
126 bp
Sequences
Splice sites
3' ss Seq
TTATTCGTTTACCCAATAAGCTG
3' ss Score
2.15
5' ss Seq
CAAGTAATA
5' ss Score
1.48
Exon sequences
Seq C1 exon
TGACTCCGCCGGCACAGCGAGTTCAATTCATACTTGGCGAGGATGTGGACGATGGCACACACGTATCGCATCCCCTGTTTTCGGAAATGGGGATGCTGGTCAAGGAGGGCGACGAGATCGAGTGGAAGGAGACAGCGCGATGGATCAAATTCGAGGAGGATGTGGAGGAGGGTGGCAATCGATGGTCGAAGCCGCACGTGGCCACGCTCTCCCTGCACTCCCTGTTCGAGCTGCGTCGCCTGCTGGTCAATGGCAGCGTGATGCTGGACATGGAGGCCCAAAATCTGGAGGTGATGGCCGATCTCGTGTGCGATCACATGGTCAGTGCGGGAACATTGCCGCCCGGTGTAAAGGATAAGGTCAAGGACGCGCTCCTGCGACGACATCGGCATCAGCACGAGTATGCCAAAAAGACGCGACTGCCAATTATTCGATCCCTGGCCGATATGCGCAATCACTCGTCATCAAAAA
Seq A exon
CTGACACTTCAACGCATCTGGGGGCCATCGGGGTCACCACCTGGTTCCATGCTGGGGCATCGCCCCAACATCAGGCACAGAATCATGCTCAGAATCAAGGACGACCGCAGTCACAAGCAATACCAA
Seq C2 exon
AGAAAAAATCTAATTCTAAACACTCGCGACCCGCACAAAACCTGTCATCGATCACAGAGGACATGGTCAAGAGTCCCAGTAACCAGTCGATGGCCAGGCCAGGAAGCGGAACGGAACTCAGCGAGCAGCAGCACAAGGGCAACACCCACTTCATGAGGAAGATACCGCCGGGCGCGGAGGCCAGCAACATTCTCGTGGGCGAGGTTGATTTCCTGGAGCGCACCCTGTCCTGTTTCATCCGTCTGAGCCAGGCGGTCGTCCTGGGCGATCTCACCGAGGTGCCAGTGCCCACAAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0259111_MULTIEX1-2/9=C1-4
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.326 A=0.674 C2=0.538
Domain overlap (PFAM):
C1:
PF075658=Band_3_cyto=PU(38.7=65.8)
A:
PF075658=Band_3_cyto=FE(14.4=100)
C2:
PF075658=Band_3_cyto=FE(36.4=100)
Main Inclusion Isoform:
FBpp0304052
Main Skipping Isoform:
FBpp0288814
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
FBpp0288812, FBpp0288813, FBpp0304048, FBpp0304049, FBpp0304050, FBpp0304051, FBpp0304053, FBpp0304054
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCGCAATCACTCGTCATCAA
R:
CTCCAGGAAATCAACCTCGCC
Band lengths:
242-368
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)