DmeEX0005821 @ dm6
Exon Skipping
Gene
FBgn0003137 | Ppn
Description
The gene Papilin is referred to in FlyBase by the symbol DmelPpn (CG33103, FBgn0003137). It is a protein_coding_gene from Dmel. It has 5 annotated transcripts and 5 polypeptides (4 unique). Gene sequence location is 3R:28520665..28540324. Its molecular function is described by: serine-type endopeptidase inhibitor activity; peptidase activity; extracellular matrix structural constituent. It is involved in the biological process described with: extracellular matrix organization. 9 alleles are reported. The phenotypes of these alleles manifest in: embryonic/larval tracheal system; embryonic/larval somatic muscle; Malpighian tubule. The phenotypic classes of alleles include: lethal - all die before end of embryonic stage; lethal - all die before end of pupal stage; some die during embryonic stage; some die during pupal stage; partially lethal; viable. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed within 12-24 hour embryonic stages.
Coordinates
chr3R:28522695-28526050:-
Coord C1 exon
chr3R:28525871-28526050
Coord A exon
chr3R:28524274-28525307
Coord C2 exon
chr3R:28522695-28523260
Length
1034 bp
Sequences
Splice sites
3' ss Seq
CAATCCCTCAACCCAACTAGCTA
3' ss Score
0.67
5' ss Seq
CCGGTTAGA
5' ss Score
3.29
Exon sequences
Seq C1 exon
CTACCTGTGAACAACCTGTGGAGAGCGGACCATGTGCCGGAAACTTCGAGCGTTGGTACTACGACAACGAGACGGACATCTGCCGACCCTTCACCTATGGTGGATGCAAGGGCAACAAGAACAACTATCCCACGGAACATGCCTGCAACTACAACTGCCGCCAGCCCGGCGTGCTTAAAG
Seq A exon
CTAAGGACATCTGTGAGATTCCGGCCGAGGTGGGCGAGTGTGCCAACTACGTTACCAGCTGGTACTACGACACTCAGGATCAGGCCTGTCGCCAGTTCTACTACGGCGGCTGTGGGGGCAATGAGAACCGCTTCCCCACCGAGGAGTCCTGTTTGGCGCGTTGCGACCGCAAGCCCGAACCCACAACCACCACCCCCGCCACCAGGCCACAACCCAGTAGGCAGGACGTCTGTGACGAGGAACCCGCTCCAGGAGAATGTAGTACCTGGGTCCTCAAGTGGCACTTCGATCGGAAGATTGGGGCTTGCCGTCAGTTCTACTATGGCAACTGTGGCGGCAATGGCAATCGCTTCGAGACAGAGAACGATTGTCAGCAGCGTTGTCTTAGCCAAGAGCCTCCTGCCCCGACTCCTCCACGAGCACCTGCTCCAACCAGGCAGCCAGATCCGGCTCCTACTGTCGCTCAATGCAGTCAGCCTGCTGATCCAGGACAGTGCGACAAATGGGCGCTCCACTGGAACTACAACGAGACCGAGGGTCGCTGTCAGTCGTTCTACTACGGCGGCTGTGGCGGCAATGATAACCGCTTTGCCACCGAGGAGGAGTGCTCTGCTCGCTGCTCTGTCAACATAGACATCCGCATTGGTGCCGATCCCGTGGAGCACGACACGTCCAAGTGCTTCTTGGCCTTCGAACCGGGTAACTGCTACAACAACGTGACCCGCTGGTTCTACAACAGCGCGGAGGGACTCTGCGACGAGTTCGTCTACACGGGCTGCGGCGGTAATGCCAACAACTATGCCACCGAGGAGGAGTGCCAGAACGAGTGCAACGACGCCCAGACCACATGTGCACTGCCTCCGGTCCGTGGACGATGCAGTGACCTCTCCCGGCGCTGGTACTTCGATGAGCGCAGCGGAGAATGCCACGAGTTCGAGTTCACTGGATGCCGCGGTAATCGAAACAACTTCGTGTCGCAGAGCGATTGCCTAAACTTCTGCATTGGGGAGCCTGTAGTGGAGCCTTCAGCGCCG
Seq C2 exon
CGGAACCCAACAAGCAGGACAACGAAATTGGACAAAATCCGTGCGATACCTTCGATGCGGAGTGCCAGGAGCTCCGCTGTCCATACGGCGTTCGCCGTGTGGCCGCCAGGTCCCAGCCAGAGTGCACGCAGTGCATCTGCGAGAATCCGTGCGAGGGCTACAGTTGCCCGGAGGGCCAACAGTGCGCCATCGATGTGGCCAGTTCCGACGATCGCCAGTTTGCTCCAGTGTGTCGCGATATTTATAAGCCAGGAGAGTGCCCGGCTCTTTCAGCGAATGCCAGTGGATGTGCCCGGGAATGCTACACCGATGCCGACTGTCGTGGAGACAACAAGTGCTGCAGCGACGGCTGCGGACAGCTGTGTGTGCATCCTGCTCGTCCAACTCAGCCGCCCAGGACTCAGGCTCCAGTGGTCTCCTATCCGGGCGATGCCAGAGCCGCTCTGGAACCCAAGGAGGCGCATGAGCTTGATGTCCAGACAGCCATCGGCGGCATAGCC
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0003137_MULTIEX1-2/5=C1-C2
Average complexity
C3
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.139 C2=0.094
Domain overlap (PFAM):
C1:
PF0001418=Kunitz_BPTI=WD(100=86.9)
A:
PF0001418=Kunitz_BPTI=WD(100=15.4),PF0001418=Kunitz_BPTI=WD(100=15.4),PF0001418=Kunitz_BPTI=WD(100=15.4),PF0001418=Kunitz_BPTI=WD(100=15.4),PF0001418=Kunitz_BPTI=WD(100=15.4)
C2:
PF0767911=I-set=PU(41.9=19.0)
Main Inclusion Isoform:
FBpp0291051
Main Skipping Isoform:
FBpp0289566
Other Inclusion Isoforms:
FBpp0291052, FBpp0291053
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTATCCCACGGAACATGCCTG
R:
CCACTGGCATTCGCTGAAAGA
Band lengths:
342-1376
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)