DmeEX0006539 @ dm6
Exon Skipping
Gene
FBgn0010905 | Spn
Description
The gene Spinophilin is referred to in FlyBase by the symbol DmelSpn (CG16757, FBgn0010905). It is a protein_coding_gene from Dmel. It has 12 annotated transcripts and 12 polypeptides (9 unique). Gene sequence location is 3L:2505245..2554292. Its molecular function is described by: neurexin family protein binding; actin filament binding. It is involved in the biological process described with: neuron projection development; negative regulation of presynaptic active zone assembly; calcium-mediated signaling; actin filament organization. 29 alleles are reported. The phenotypes of these alleles manifest in: presynaptic active zone; embryonic/larval neuromuscular junction. The phenotypic classes of alleles include: smell perception defective; behavior defective; viable; flightless; neuroanatomy defective; partially lethal - majority die. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages.
Coordinates
chr3L:2513696-2525718:-
Coord C1 exon
chr3L:2524599-2525718
Coord A exon
chr3L:2514310-2514330
Coord C2 exon
chr3L:2513696-2514197
Length
21 bp
Sequences
Splice sites
3' ss Seq
CCTGTATCCTGTCTGCGTAGCAT
3' ss Score
6.68
5' ss Seq
CAGGTTGGT
5' ss Score
8.08
Exon sequences
Seq C1 exon
GTTGGTGGTGGAGGCAGTGCCAAAACCACGCCCCAATGCTCTCCCGCCCGTTCGGCCTTAGTAAAGGGCATTGCCCCCATTGCTTCGATCGATGCGCACGAAGAGGAGGAACTAGATCTCGTAGAGGAGGACGAGCACCTGGCCGTGGAGTATGTGGAGGTGCTGGAACTCCAGCAGGACGAAGAGGAGGAAGAGGCTCCGGTCCTTCCCGAGCGACGAGCTCCGGCTCAAGGCTCCCTTGAGCTTCAGGACTTGGAGTATGCCGATACCAGCGCTGGCGAGGATGAGGAGGACATCATAAACCATCTAAAGGACGGCGATGTATTGGATGTGGAACTAATCGACGATGTCGTGGATGAGGTGATCAAGGTTCATGTGAATCACAGTGTGGCCACTGCCCCTTCGATTCAAGCTGCTACACCAGCTGCAGCGATTCCACGGGAGGATAGCCTGCCGGATGACATGACCGCCGCCGAGGCCGAAAGATTGTTGAGCTCTAG
Seq A exon
CATTTTGGAAAACAAAATCAG
Seq C2 exon
ACAACAGTCGCTGCTGTCGGACGAACAGGCCAAGGAAGTCGAGCAAATACTCAACGCCGCCCCCAGCGTGGGCGTGGCAGTTGCTACCGTTGTTGCCACAGCAACATCGCCGACCAGCATCAAGAATCTCATTGAGGATTTGCCCGGGCAATCGGCAGTCGCCGCCAGCGCTGCCAACGGGGAGCAGGACATTCAAATCGCAGCTGTCCCAGCAATTGTCGAGGAGGACGAGGACGAAGAGGAGGACTTCCCGGAGGACGACGAGGAGGACCACGCCAGGGCCGATTTTGATGCCAACGGCGGTGACGCTGACGGCGATTCCGATGACGTTGAAGCCGTGGACATTGTGGGCTATGGCCATGCATCCACAGCTCTGAATGCCACCTTCGTCAAGGCCGACAGCACAGAGACAGAGACAACCACCACTACACCTTCGACAGCAACCACAGCCACCACTCGCCACGACGACGACGAGCCCGAGTGGCTAAGGGATGTCCTAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0010905_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.838 A=0.000 C2=0.766
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Inclusion Isoform:
FBpp0293163
Main Skipping Isoform:
FBpp0293160
Other Inclusion Isoforms:
FBpp0293155, FBpp0293156, FBpp0293157, FBpp0293158, FBpp0293159, FBpp0293161, FBpp0293162, FBpp0311289, FBpp0311290
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATAGCCTGCCGGATGACATG
R:
ATTTGCTCGACTTCCTTGGCC
Band lengths:
104-125
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)