DmeEX0007443 @ dm6
Exon Skipping
Gene
FBgn0000289 | cg
Description
The gene combgap is referred to in FlyBase by the symbol Dmelcg (CG8367, FBgn0000289). It is a protein_coding_gene from Dmel. It has 12 annotated transcripts and 12 polypeptides (10 unique). Gene sequence location is 2R:14173693..14182838. Its molecular function is described by: DNA binding; DNA-binding transcription factor activity. It is involved in the biological process described with: regulation of transcription, DNA-templated; imaginal disc-derived wing morphogenesis. 41 alleles are reported. The phenotypes of these alleles manifest in: adult antennal segment; adult central brain; embryonic/larval digestive system; alimentary canal; imaginal tissue. The phenotypic classes of alleles include: partially lethal; increased mortality; phenotype; increased mortality during development. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages, in adult female stages.
Coordinates
chr2R:14179313-14182838:+
Coord C1 exon
chr2R:14179313-14179931
Coord A exon
chr2R:14180422-14180819
Coord C2 exon
chr2R:14181211-14182838
Length
398 bp
Sequences
Splice sites
3' ss Seq
CCTTCTCCAATACTTTGCAGCTT
3' ss Score
7.7
5' ss Seq
TAGGTGTGT
5' ss Score
4.3
Exon sequences
Seq C1 exon
TCTTGAGCACGGCTAGCCTGAATCCGGGCACCGTGGTCACGGCCAATCCGAATCTGGTGGGCCAGAGCCCTGTGCCAGTGCAGATCATATCGTCCACCGGTCAGGTGATGTCGCAGACCACGCTGGTGCAGGCCGCCAACTCGACCCATCCACAAGCCGTGGTCACCGCTGTGCCTACGATGCCCGTGCATGGTCAGCAGCAGCATCTGCAGCATGTGGCCCAACAGCAGCAGCAACAGCAACAACAGCAGCAACAGCAGCAGCAACATGTTGTCTCCGTGGCGCCGGCCAACAAACCGAAGTCGCATTTCTGCGCCAGCTGCGGCAAGGGATTCGCCGCCAAGCACGGTCTCATGCAGCACAATCGGCGTCACCCGAACGGCGGCTGCACGGTGCGCACACACGTGTGCGAGTGCGGCAAGGCCTTCTTCCAGAAGAACCACCTGATGCTGCACCAGCGCCAGCATCTGGAGACGAAGCCAGCCATATCGCAGCAACAG
Seq A exon
CTTGTTGTACCAACTACTTCTTACTAAGAATTAAACGAAACAGAAAAACTGAAAAAAAAAATGATAAATACAACCACAAATGAACATTAAAACCTAATCAAACGAAAATCAACTCGAGCAACATCCATTTGCACAAGCGCACACCCACATGTTTGAATGTAAATTCAAACTCAGATGCGAGAGGAACTGGAGATTTTTGATCCTTTCCGTTCGGATTTGGGTGAGATGTGTGTGCACGTGTGCGCGGGTGTGTCTGCCTATGTGAAGGTGTGTCTGTCTATGCGTGTCTCTTATCAAAATCTATAAACTAAAAGAAACCCATAACACTGCAATGAATACAATCTAATCGTATAGTATCAAATCGAATTCCATTCAAAGGCTTCAGTTTGCGCGTTTAG
Seq C2 exon
GAGGCCGCCACCCAGCAGCAACTGGCGACTGCCGGCGAGCAGCAGCAGGTACAGGTGCAGATAATGCCTGATGGCCAGATTCACGGAAAAGTAATCAAGTACGAGATCTGTCGCAATGTAATGCCGGAGGATCAGCAGCAGGAGCAGGCGGACATGAACGCGGAGTAATTCAGATAAACTGTAAATACATGGGACGTGCAACTGCAACAGCAGCAACACAAAAGTAGCAACAGCATCAGTAGCAACATCATCAGTAGCAACAGCAACAGCATCAGCGCATAATGGGACACCAGCACAGTTGGACGGAGACAGGCAGATACCCACCAGAAAAAGGTCATCGGAGTGGGGCGTATGCATCGTAAGCGATTCCCTTAAATGTAATATTTTAGTTAGCCTTACGTTAGCGGTTAAGTGATCGGATTGTACACATAAATGTTAACTAGCGTTACACACATGAACACACCTTATATAATATATATATACAGACATATATAGGTTTA
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0000289_CASSETTE3
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.478 A=0.000 C2=0.636
Domain overlap (PFAM):
C1:
PF0009621=zf-C2H2=PD(82.6=9.0)
A:
NO
C2:
NO
Main Inclusion Isoform:
FBpp0111707
Main Skipping Isoform:
FBpp0308983
Other Inclusion Isoforms:
FBpp0312412
Other Skipping Isoforms:
FBpp0288474
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAAGAACCACCTGATGCTGCA
R:
TCCCATTATGCGCTGATGCTG
Band lengths:
354-752
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)