Special

DmeEX0008775 @ dm6

Exon Skipping

Gene
FBgn0266347 | nAChRalpha4
Description
The gene nicotinic Acetylcholine Receptor alpha4 is referred to in FlyBase by the symbol Dmel AChRalpha4 (CG12414, FBgn0266347). It is a protein_coding_gene from Dmel. It has 7 annotated transcripts and 7 polypeptides (5 unique). Gene sequence location is 3L:23206669..23287448. Its molecular function is described by: neurotransmitter receptor activity; acetylcholine-gated cation-selective channel activity; acetylcholine receptor activity. It is involved in the biological process described with 6 unique terms, many of which group under: multicellular organismal process; cellular process; biological regulation; signaling; chemical synaptic transmission. 13 alleles are reported. No phenotypic data is available. The phenotypic classes of alleles include: sleep defective; viable; fertile; partially lethal - majority die; memory defective.
Coordinates
chr3L:23227438-23262823:+
Coord C1 exon
chr3L:23227438-23227547
Coord A exon
chr3L:23238837-23239017
Coord C2 exon
chr3L:23262708-23262823
Length
181 bp
Sequences
Splice sites
3' ss Seq
ATACTGAAAATATCATGTAGTGC
3' ss Score
0.98
5' ss Seq
AAGGTAGGT
5' ss Score
10.29
Exon sequences
Seq C1 exon
TCGTGGTATGATTACAAACTTCGTTGGGAACCTAAAGAATATGGTGGAGTTCATATGCTGCATGTTCCTTCAGATCATATATGGAGACCGGATATTGTCCTTTATAATAA
Seq A exon
TGCTGATGGTAACTTCGAAGTGACTCTTGCAACTAAAGCCACAATTTACTCAGAAGGATTAGTTGAATGGAAGCCACCAGCAATATACAAATCATCCTGTGAAATAGATGTAGAATATTTCCCATTTGATGAACAGACCTGTGTTCTAAAATTTGGCAGTTGGACTTATGATGGGTTTAAG
Seq C2 exon
GTTGATCTGAGGCACATGGATGAACAACAGGGCAGCAACGTTGTTGCTGTGGGTGTGGACCTATCTGAATTTTATATGTCTGTGGAATGGGACATTCTCGAAGTTCCAGCTGTTAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0266347_MULTIEX1-3/11=1-6
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.000 A=0.000 C2=0.000
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0293118=Neur_chan_LBD=FE(16.7=100)

							
A:
PF0293118=Neur_chan_LBD=FE(27.8=100)

							
C2:
PF0293118=Neur_chan_LBD=PU(55.9=97.1)

						

					

				








    
Main Inclusion Isoform:
FBpp0289395





     
                   









    
Main Skipping Isoform:
FBpp0312320





     
      









    
Other Inclusion Isoforms:
FBpp0305094
          









    
Other Skipping Isoforms:
NA
  



Associated events







Conservation


Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
TCGTGGTATGATTACAAACTTCGT

				
R: 
CTAACAGCTGGAACTTCGAGA

				
Band lengths: 
226-407

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Neural diversity
  • Neurogenesis
  • Neuronal activity
  • Splicing factor regulation (brain)
  • Splicing factor regulation (SL2)





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