DmeEX0008777 @ dm6
Exon Skipping
Gene
FBgn0266347 | nAChRalpha4
Description
The gene nicotinic Acetylcholine Receptor alpha4 is referred to in FlyBase by the symbol Dmel
AChRalpha4 (CG12414, FBgn0266347). It is a protein_coding_gene from Dmel. It has 7 annotated transcripts and 7 polypeptides (5 unique). Gene sequence location is 3L:23206669..23287448. Its molecular function is described by: neurotransmitter receptor activity; acetylcholine-gated cation-selective channel activity; acetylcholine receptor activity. It is involved in the biological process described with 6 unique terms, many of which group under: multicellular organismal process; cellular process; biological regulation; signaling; chemical synaptic transmission. 13 alleles are reported. No phenotypic data is available. The phenotypic classes of alleles include: sleep defective; viable; fertile; partially lethal - majority die; memory defective.
Coordinates
chr3L:23227438-23262823:+
Coord C1 exon
chr3L:23227438-23227547
Coord A exon
chr3L:23251453-23251579
Coord C2 exon
chr3L:23262708-23262823
Length
127 bp
Sequences
Splice sites
3' ss Seq
CATATTTTCCGTTCTTACAGATC
3' ss Score
11.19
5' ss Seq
AAAGTAAGA
5' ss Score
6.91
Exon sequences
Seq C1 exon
TCGTGGTATGATTACAAACTTCGTTGGGAACCTAAAGAATATGGTGGAGTTCATATGCTGCATGTTCCTTCAGATCATATATGGAGACCGGATATTGTCCTTTATAATAA
Seq A exon
ATCCGCCATGTTTGTTTTTTGGCTGACCCTGCTTCTCCATGGATCACTTTGCTGTAAACTATCGTGGACTTTAAGTCATTTGTGAAAACCACTACACCAAAACTTCTTGCATCGCAGCAGTCCAAAA
Seq C2 exon
GTTGATCTGAGGCACATGGATGAACAACAGGGCAGCAACGTTGTTGCTGTGGGTGTGGACCTATCTGAATTTTATATGTCTGTGGAATGGGACATTCTCGAAGTTCCAGCTGTTAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0266347_MULTIEX1-5/11=1-6
Average complexity
C3*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0293118=Neur_chan_LBD=FE(16.7=100)
A:
NA
C2:
PF0293118=Neur_chan_LBD=PU(55.9=97.1)
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0289395
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
FBpp0305094, FBpp0312320
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCGTGGTATGATTACAAACTTCGT
R:
CTAACAGCTGGAACTTCGAGA
Band lengths:
226-353
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)