DmeEX0009544 @ dm6
Exon Skipping
Gene
FBgn0032901 | sky
Description
The gene skywalker is referred to in FlyBase by the symbol Dmelsky (CG9339, FBgn0032901). It is a protein_coding_gene from Dmel. It has 13 annotated transcripts and 13 polypeptides (7 unique). Gene sequence location is 2L:20871335..20918931. Its molecular function is described by: GTPase activity; GTPase activator activity. It is involved in the biological process described with 7 unique terms, many of which group under: signaling; anterograde trans-synaptic signaling; synaptic signaling; developmental process; negative regulation of signaling. 56 alleles are reported. The phenotype of these alleles manifest in: retina. The phenotypic classes of alleles include: phenotype; increased mortality; lethal - all die before end of pupal stage; increased mortality during development. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, during late larval stages, during late pupal stages, in stages of adults of both sexes.
Coordinates
chr2L:20874274-20877083:-
Coord C1 exon
chr2L:20876912-20877083
Coord A exon
chr2L:20876587-20876599
Coord C2 exon
chr2L:20874274-20874432
Length
13 bp
Sequences
Splice sites
3' ss Seq
CAGAAGCTGCACTCCGAGAGCTG
3' ss Score
-9.95
5' ss Seq
TTGGTATGA
5' ss Score
5.24
Exon sequences
Seq C1 exon
TACCCAGTATATTTCTAGAATATTTATCAAGACTGAAATGCTACTAAAAAGCCGCTCCGTGCTCACCAGCGGTTCGAAGCAATTAATCAAATCGCGTTCAAGTGATAATCTGCCCACTAGTCAGTCGCAGGTCAACATCCAAATGATGTCCCACACCCTGACCATCCGAGAG
Seq A exon
CTGTCGCAATTTG
Seq C2 exon
CTGTTCACGCTGTGGTCCTGGCTGCCCGTGCGGATAACAATGTACCAACCGGTGCTGCTCTACACCACGGAGGAGCACGGCTGCTCACTAACCACGTTCTACGTTCGGGTGGAGCAGCATGAACCCACGCTGCTCATGATCAAGACGTGCAATAATGAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0032901-MICROEX1
Average complexity
MIC-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.025 A=0.029 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0753411=TLD=PU(22.2=64.2)
Main Inclusion Isoform:
FBtr0081440fB1073

Main Skipping Isoform:
FBpp0080974

Other Inclusion Isoforms:
FBpp0303287, FBpp0303289
Other Skipping Isoforms:
FBpp0080969, FBpp0080970, FBpp0080971, FBpp0080972, FBpp0080973, FBpp0080975, FBpp0080976, FBpp0291173, FBpp0303288
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAGTCGCAGGTCAACATCCAA
R:
TACATTGTTATCCGCACGGGC
Band lengths:
95-108
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)