Special

DmeEX0009545 @ dm6

Exon Skipping

Gene
Description
The gene skywalker is referred to in FlyBase by the symbol Dmelsky (CG9339, FBgn0032901). It is a protein_coding_gene from Dmel. It has 13 annotated transcripts and 13 polypeptides (7 unique). Gene sequence location is 2L:20871335..20918931. Its molecular function is described by: GTPase activity; GTPase activator activity. It is involved in the biological process described with 7 unique terms, many of which group under: signaling; anterograde trans-synaptic signaling; synaptic signaling; developmental process; negative regulation of signaling. 56 alleles are reported. The phenotype of these alleles manifest in: retina. The phenotypic classes of alleles include: phenotype; increased mortality; lethal - all die before end of pupal stage; increased mortality during development. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, during late larval stages, during late pupal stages, in stages of adults of both sexes.
Coordinates
chr2L:20874274-20877083:-
Coord C1 exon
chr2L:20876912-20877083
Coord A exon
chr2L:20875080-20875136
Coord C2 exon
chr2L:20874274-20874432
Length
57 bp
Sequences
Splice sites
3' ss Seq
CCATCTATATCTTTTCATAGCAT
3' ss Score
6.72
5' ss Seq
TTTGTAAGC
5' ss Score
3.93
Exon sequences
Seq C1 exon
TACCCAGTATATTTCTAGAATATTTATCAAGACTGAAATGCTACTAAAAAGCCGCTCCGTGCTCACCAGCGGTTCGAAGCAATTAATCAAATCGCGTTCAAGTGATAATCTGCCCACTAGTCAGTCGCAGGTCAACATCCAAATGATGTCCCACACCCTGACCATCCGAGAG
Seq A exon
CATTTTGGTTTGCCGGGCACAAAGAATTTCATAAAAACCTGGACGGATAGACAATTT
Seq C2 exon
CTGTTCACGCTGTGGTCCTGGCTGCCCGTGCGGATAACAATGTACCAACCGGTGCTGCTCTACACCACGGAGGAGCACGGCTGCTCACTAACCACGTTCTACGTTCGGGTGGAGCAGCATGAACCCACGCTGCTCATGATCAAGACGTGCAATAATGAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0032901_MULTIEX1-3/3=C1-C2
Average complexity
ME(1-3[99=100];2-3[97=100])
Mappability confidence:
75%=75=100%
Protein Impact

Alternative protein isoforms (Ref)

No structure available
Features
Disorder rate (Iupred):
  C1=0.025 A=0.000 C2=0.000
Domain overlap (PFAM):

C1:
NO
A:
NO
C2:
PF0753411=TLD=PU(22.2=64.2)


Main Inclusion Isoform:
FBpp0080973


Main Skipping Isoform:
FBpp0080974


Other Inclusion Isoforms:
FBpp0080970, FBpp0080971, FBpp0080976


Other Skipping Isoforms:
FBpp0080969, FBpp0080972, FBpp0080975, FBpp0291173, FBpp0303287, FBpp0303288, FBpp0303289
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCAAATGATGTCCCACACCCT
R:
AGAACGTGGTTAGTGAGCAGC
Band lengths:
134-191
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Neural diversity
  • Neurogenesis
  • Neuronal activity
  • Splicing factor regulation (brain)
  • Splicing factor regulation (SL2)