DmeEX0010115 @ dm6
Exon Skipping
Gene
FBgn0010328 | woc
Description
The gene without children is referred to in FlyBase by the symbol Dmelwoc (CG5965, FBgn0010328). It is a protein_coding_gene from Dmel. It has 6 annotated transcripts and 6 polypeptides (5 unique). Gene sequence location is 3R:27256595..27264055. Its molecular function is described by: zinc ion binding; chromatin binding; protein binding; DNA-binding transcription factor activity, RNA polymerase II-specific. It is involved in the biological process described with: ecdysone biosynthetic process; regulation of transcription, DNA-templated; regulation of transcription by RNA polymerase II. 30 alleles are reported. The phenotypes of these alleles manifest in: circulatory system; cellular anatomical entity; organism subdivision; intracellular non-membrane-bounded organelle; intracellular organelle. The phenotypic classes of alleles include: lethal; lethal - all die before end of pupal stage; phenotype; increased mortality during development. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages.
Coordinates
chr3R:27260091-27262487:-
Coord C1 exon
chr3R:27262227-27262487
Coord A exon
chr3R:27262116-27262160
Coord C2 exon
chr3R:27260091-27261943
Length
45 bp
Sequences
Splice sites
3' ss Seq
TCTATCCATTTTGCCTGCAGATG
3' ss Score
10.52
5' ss Seq
AAGGTGAGC
5' ss Score
9.6
Exon sequences
Seq C1 exon
ACACAGTGGAAAATGTTTTGGAGCCAGAGGAATCAGATGTTTGCCTTATTCCCGATGATCAAGAAACGGAAGTGACTGAAGCTGAGAAGGAACTGGCCCGCGAGAATGCCGAGAAGGCCGCCGAAGAGGAGGAAGCCGAGGAGCGGCAAGCCAAGACACCCGATAACGATAGAAAGTCCCCGGCAGATGCCGCCGCCGATCCAGAAGACGACAGTGCAGCTGCCGTTGACGCCACCGACGTATCTCCAACCACGCCAACTG
Seq A exon
ATGCTCAAGCTGGAGCTAAGTCGGCGGCAACAGGCATTGCAGAAG
Seq C2 exon
CAGATGGAGTAGCTGCAGCAGCTAACGAAGAGGAGGCCGGTGGCGATGTTCCCGATCCCGACGAGCCCACAGCCGAGGAATCCTCCAGTGCCGGAGCGGGTGAACCTGGTTCCACGCCGAAAATCATCATTAGTTGGATCGTTGGCAGCGTTCAGAAGTGCAAACAGTGTGGCGATGAAAAGAATTGTGGGTTTCGGTACAGGAGTCCAGAGGAGTCAGAAGAAATGGATAAGGAGAAGGAAAACGATAAGGCAGAGGAGAACGCTGAGGAGGGGGAGGAGGAGGAGGCTCGGCAAAAGCCTGCTGCTCCCAGTGGGTTCGAGTACATCTGCGATACCGCCTGTGTCGACGCCCTGCTAGAAGATCAACCCGGCAAGTACTTTGTACGCCGCAAGAAGTTCCTGGTCGAGGAAGTGGTTCGAGAAGAAGGCGCCGCCGAAGCGGAGGCTAGCGCCGATGGCGAGGGCGGAGATGCCGAGGAGGAAGCTACTCCAGCCAAC
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0010328-'5-4,'5-3,6-4
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=1.000 C2=0.281
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF064679=zf-FCS=WD(100=6.8),PF064679=zf-FCS=WD(100=6.8),PF064679=zf-FCS=WD(100=7.1)
Main Inclusion Isoform:
FBpp0111770
Main Skipping Isoform:
FBpp0084548
Other Inclusion Isoforms:
FBpp0306779
Other Skipping Isoforms:
FBpp0306778
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCAAGCCAAGACACCCGATAA
R:
CTGCTGCAGCTACTCCATCTG
Band lengths:
137-182
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)