DmeEX6007361 @ dm6
Exon Skipping
Gene
FBgn0264695 | Mhc
Description
The gene Myosin heavy chain is referred to in FlyBase by the symbol DmelMhc (CG17927, FBgn0264695). It is a protein_coding_gene from Dmel. It has 22 annotated transcripts and 22 polypeptides (20 unique). Gene sequence location is 2L:16766566..16788766. Its molecular function is described by 6 unique terms, many of which group under: nucleoside-triphosphatase activity; catalytic activity; hydrolase activity, acting on acid anhydrides; drug binding; actin filament binding. It is involved in the biological process described with 15 unique terms, many of which group under: epithelial cell development; adult somatic muscle development; regulation of biological quality; reproductive process; protein stabilization. 161 alleles are reported. The phenotypes of these alleles manifest in: somatic cell of ovariole; muscle attachment site; embryonic/larval heart; adult visceral muscle; germline cell. The phenotypic classes of alleles include: sterile; fertile; phenotype; increased mortality during development.
Coordinates
chr2L:16785909-16788766:+
Coord C1 exon
chr2L:16785909-16786814
Coord A exon
chr2L:16787079-16787580
Coord C2 exon
chr2L:16788021-16788766
Length
502 bp
Sequences
Splice sites
3' ss Seq
GCTTCCACTTACGAAAACAGATC
3' ss Score
3.52
5' ss Seq
TTTGTAAGT
5' ss Score
7.4
Exon sequences
Seq C1 exon
GCGCTGAGCAGGATCATGCCCAGACCCAGGAGAAATTGAGGAAGGCCCTCGAGCAGCAGATCAAGGAGCTGCAGGTCCGTCTGGACGAGGCTGAGGCCAACGCCCTCAAGGGAGGCAAGAAGGCCATTCAGAAGCTTGAGCAGCGCGTCCGCGAGCTCGAGAACGAGCTGGATGGTGAGCAGAGGAGGCACGCCGATGCCCAGAAGAACCTGCGCAAGTCCGAGCGTCGCGTCAAGGAGCTGAGCTTCCAGTCCGAGGAGGACCGCAAGAACCACGAGCGCATGCAGGATCTGGTCGACAAGCTGCAACAGAAGATCAAGACATACAAGAGGCAGATCGAGGAGGCTGAGGAAATCGCCGCCCTCAACTTGGCCAAATTCCGCAAGGCTCAGCAGGAGCTTGAGGAGGCCGAGGAGCGCGCCGATCTGGCCGAGCAGGCCATCAGCAAATTCCGCGCCAAGGGACGTGCCGGTTCTGTCGGTCGTGGTGCCAGCCCAGCG
Seq A exon
ATCTAAGCCTGAGGCATCACCATCGAATAAAAAATCTATATAGCGATCCAATTATGGTTCATTTACGATAATGAGAGAACAAAAACCATGCAAAGAGAATTATAGCTTATAAGAATTATTATAAAATAAATACTAATAACAATAATATAGATGCATCACATGACATTCGCTAGCAGACAAAAATGTATAAATTAGAGGCCACCCCAATCAAAAATCAATGGATCAATTCATCGAATACCAACCAAGAATGCGTTCATTTTGACAGAAAACCAGACAAAAAGCCAAAAAAATATTGCAAATAAAAAAGCAAGCAACCAACGCAGCACCAGGCTAAATCAATCGAAATGCTTTCTTTACCACTATTTACAAAAAAGAGAAAAAACAATACTATTCTTCCACTATATTATTATAAACTGTATTTGTACATGTGTACTGCAGCCGACAGCGTCAAAGGGCCGCAAGAGCGCGCTGCTGGAGCAGTAGAAACTTTCTTGAAACATTT
Seq C2 exon
CCCCGTGCGACGTCCGTTAGGCCACAATTCGACGGATTGGCCTTCCCACCAAGATTCGACCTTGCTCCTGAAAACGAATTCTAAATGCCATTTCATTTTTTAATTTATTTTAAATGATATTTCATAATGATTATGTTTATGATTTTAATTTAATTTCTTAATTTAAAAACAAAATAATAAAACTATAACAAAATAAATATCGAAAACGACGGGAGGCAAACCACCAACAACGCCAGATGCACTTAGGCAAAAAAATAAAATCATATAACAACAATCGATCACCATCGATTAGCATACATAATACATAAGTGATCAGTAACAGGCTATTGTGTAAGCGCGACATTTGCCATGGGGACACTGCTCGCTGGACCTGTATTTGTATTTGTATTTATATATTTTTATACAACTTTCGAAATGCATTCAACCAACTAACTATGAAAGAACCATCGACTCTCAACCGAACAACATGAGAATAATCGCGAAACGTGAGCCCCGTCAAA
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0264695-'42-44,'42-40,43-44=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.801 A=1.000 C2=0.556
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=PD(33.9=96.4),PF131661=AAA_13=PD(15.5=9.3)
A:
NO
C2:
NO
Main Inclusion Isoform:
FBpp0080462
Main Skipping Isoform:
FBpp0080452
Other Inclusion Isoforms:
FBpp0080463, FBpp0080464, FBpp0291041, FBpp0291043, FBpp0298831
Other Skipping Isoforms:
FBpp0080453, FBpp0080454, FBpp0080455, FBpp0080456, FBpp0080457, FBpp0080458, FBpp0080459, FBpp0080460, FBpp0291042, FBpp0298827, FBpp0298828, FBpp0298829, FBpp0298830, FBpp0312323
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCAAGGAGCTGAGCTTCCAGT
R:
CGTTTTCAGGAGCAAGGTCGA
Band lengths:
345-847
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)