DmeEX6023552 @ dm6
Exon Skipping
Gene
FBgn0039463 | CG18472
Description
The gene Spag1 axonemal dynein assembly factor is referred to in FlyBase by the symbol DmelSpag1 (CG18472, FBgn0039463). It is a protein_coding_gene from Dmel. It has 2 annotated transcripts and 2 polypeptides (1 unique). Gene sequence location is 3R:26947830..26949682. Its molecular function is described by: heat shock protein binding. It is involved in the biological process described with: protein targeting to mitochondrion. 6 alleles are reported. No phenotypic data is available. The phenotypic class of alleles includes: viable. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of no expression detected. Peak expression observed at stages throughout the pupal period, in adult male stages.
Coordinates
chr3R:26948724-26949682:+
Coord C1 exon
chr3R:26948724-26948851
Coord A exon
chr3R:26948911-26949038
Coord C2 exon
chr3R:26949092-26949682
Length
128 bp
Sequences
Splice sites
3' ss Seq
ATTGTTCGATTCATTCGCAGATC
3' ss Score
8.72
5' ss Seq
GAGGTAATA
5' ss Score
7.96
Exon sequences
Seq C1 exon
ACATCGACTGCGTGGCAATGAGAGCTTCAAGGCAAAGGAATATGAAAATGCCATTGAGGAGTACAACTGTTCAATAATATACGATCCGGAGAATGCAGTACACGCTTACAATAATCGCGCTGTCGCTC
Seq A exon
ATCTCAAGTTGAAGAAATACTTTTCGGCCATATCGGACTGCCAAGCCTGTCTGCAGATCGATCCAATGAATATTAAGGCACACCTTCGAATGGCTGAGGCCCACAATGCCGAAGGAAAACATCTTGAG
Seq C2 exon
TCCCTAAATGTTTACAAAAAGCTCCTCGACTTTGAGCCAGATAATGCCATTGCCAAGAAGGCCGTCGAGAAGCTAACTTCAATGCTGGGTGAGGTGGCTCCGTCCTCTGCCACACGTTTGATAATTGAGGAGATCGATCCTCCGCAACTGAAGACTTCCGAACCCAAGAAGGAGGCTGAGAAAAGTGAACCAACTGTGGTCAAGAAACCCGAACCAGTTGTCTCGGCAAAGAAGCCACCACCCATTAAAGACTACGATCTAGCCGAGTTGGTCAAGCCGAATCGCATGGTGAAAAGTAACTTGGTGTCAGCCGCAGAAGCCTTGGGAAATAAGATGCAGGCCAGCAAGGGAGGTGCACCTAAGAAGCCACAAAGTCCTCCTATGACACCAAAGGAAACCTTACTGCGTTTGCCACAGGACAACCTTAACAATAGCAATAAACTTCTTATACAGGAAATTTAGAGAACTGCATACAGTGTCTGCAGCGTGATGTTTTTACG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0039463-'2-3,'2-2,3-3=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.114 A=0.163 C2=0.641
Domain overlap (PFAM):
C1:
PF149701=DUF4509=PD(19.5=75.0),PF134141=TPR_11=FE(62.3=100)
A:
PF134141=TPR_11=PD(30.4=48.8),PF134141=TPR_11=PU(37.7=46.5)
C2:
PF134141=TPR_11=PD(58.5=20.1)
Main Inclusion Isoform:
FBpp0084440
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Chicken
(galGal3)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGGAGAATGCAGTACACGCTT
R:
GGTTCGGGTTTCTTGACCACA
Band lengths:
257-385
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)