DmeEX6024481 @ dm6
Exon Skipping
Gene
FBgn0004606 | zfh1
Description
The gene Zn finger homeodomain 1 is referred to in FlyBase by the symbol Dmelzfh1 (CG1322, FBgn0004606). It is a protein_coding_gene from Dmel. It has 4 annotated transcripts and 4 polypeptides (3 unique). Gene sequence location is 3R:30765926..30789202. Its molecular function is described by: DNA-binding transcription factor activity, RNA polymerase II-specific; RNA polymerase II regulatory region sequence-specific DNA binding. It is involved in the biological process described with 12 unique terms, many of which group under: organic cyclic compound biosynthetic process; mesoderm development; cell motility; negative regulation of muscle cell differentiation; nephrocyte differentiation. 45 alleles are reported. The phenotypes of these alleles manifest in: germline cell; somatic stem cell; trachea; A1-7 dorsal acute muscle 1; intracellular. The phenotypic classes of alleles include: female sterile; phenotype; increased mortality during development; lethal - all die before end of larval stage. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed at stages throughout embryogenesis.
Coordinates
chr3R:30773987-30789202:+
Coord C1 exon
chr3R:30773987-30774697
Coord A exon
chr3R:30783943-30785941
Coord C2 exon
chr3R:30786010-30789202
Length
1999 bp
Sequences
Splice sites
3' ss Seq
AACCTACCTCCTATTTGCAGACC
3' ss Score
8.8
5' ss Seq
CCGGTGAGT
5' ss Score
10.9
Exon sequences
Seq C1 exon
AGCTGGCCAAGAACCACAACAGTGCTAACAAAATGTCGCCGATGTGCTCACCTGGCTCCCTCACGCCCGGCGACCTCTTCGCCCAGCTGCAGCATCCGCCGCCCCAACTGCCGCCCCACCTGCACGCCCAGTTCATGGCCGCCGCCGCCTCCCTGGCCATGCAGTCCGCCCGCACCGCCAGCTCGCCCAGCCAACAGCAGCAGCAGCAACTGCAACAGCAGCAGCAACTGCAGCAGCAGCAGCAGCATCAAATGGCCATGCAGCAACTGCTGCCGCCGCAACTGCCCGGCAGCAACAGCAGCGTGGGCAGCAACTCCGCCTACGATTTGGATCTCAGCGCCCCACGCTCCACATCCAGTCCGGGCTCCACCACCGGCGACCTGAGTGGCGCCTATCCATGCATGCAGTGCACCGCCTCCTTCGCCAGCCGCGAGCAACTCGAGCAGCACGAGCAGCTCCACTCGCCATGCGGACCGGCGGCGGTCAGCAATGTCTCCCAG
Seq A exon
ACCTGCCGCATCTGCCACAAGGCCTTCGCGAACGTCTACCGCCTGCAGAGGCACATGATCAGCCACGACGAGAGTGCGCTGCTGCGGAAGTTTAAGTGCAAGGAGTGCGACAAGGCCTTCAAGTTCAAGCACCACCTCAAGGAGCACGTGAGGATCCATTCCGGCGAGAAGCCCTTCGGATGCGACAACTGCGGCAAGCGATTCTCGCACTCGGGCAGCTTCTCCTCCCACATGACCTCCAAGAAGTGCATCAGCATGGGCCTCAAGCTGAACAACAATCGCGCTCTGCTGAAGCGACTGGAGAAGAGCCCGGGATCCGCATCCTCCGCATCCCGGCGATCGCCCTCCGATCACGGAAAGGGCAAGCTGCCGGAGCAGCCGTCGCTACCGGGACTACCACATCCGATGAGCTACTTCGCCAGCGATGCGCAGGTGCAAGGTGGAAGTGCGGCACCCGCGCCCTTCCCGCCATTCCATCCGAACTACATGAACGCGGCCCTGCTCGCCTTTCCCCACAACTTTATGGCGGCTGCAGCTGGCCTGGATCCTCGGGTGCATCCCTACAGCATCCAGAGGCTGCTGCAACTTTCCGCCGCCGGTCAGCAGCAGAGGGAGGAGGAGCGTGAGGAGCAGCAAAAGCAGCAGCAGCATGATGAGGAGGAGACCCCCGATGAGCCCAAGCTAGTGATGGATATCGAGGAGCCAGAGACCAAGGAGATGGCACCCACGCCAGAGGCCACCGAAGCAGCCACTCCCATTAAGCGGGAGGAGAGCAGGGAAGCCTCTCCCGATCCAGAGAGCTACCGTTCCTCCTCGCAAGCAATCAAACAAGAGCAGGAGCCCCTGAACGTAGCAGAGGAACGTCAAACTCCTGTGGAAGAACACGCCCCCGTGGAGCATGCCGCTGATCTGCGCTGCAGTCGCTGCTCCAAGCAGTTCAATCATCCCACTGAGCTGGTGCAGCACGAGAAGGTGCTTTGTGGCCTGATCAAGGAGGAGCTGGAGCAGCACTTCCAACAGCAACAGGCCACGTCCTTTGCCTTGGCCTCCGCCAGCGAAGAGGACGAGGAAGACGAGGAGATGGATGTGGAGGAGGAGCCCCGCCAGGAGAGTGGCGAACGGAAAGTCCGCGTGCGAACGGCCATCAACGAGGAGCAGCAGCAGCAGTTGAAGCAGCACTACTCCCTGAACGCTCGACCCAGTAGGGATGAGTTCCGAATGATAGCAGCTCGTCTGCAGCTGGATCCCCGAGTGGTTCAAGTGTGGTTCCAGAACAACCGCTCCAGGGAGCGCAAAATGCAGAGTTTCCAGAACAATCAGGCGGCAGGCGCAGCACCGCCAATGCCCATTGACTCCCAGGCATCTCTAACCCGGGAGGATCAACCGCTGGACTTGTCCGTGAAGAGAGATCCCCTCACGCCGAAGAGTGAGAGCTCGCCTCCGTATATAGCTCCACCGTCGGGAGAAGCCCTCAATCCCGAAGCAATCAATCTCAGCAGGAAGTTCTCCACATCCGCATCGATGTCGCCGGCCTCGATTTCACCGTCATCCGCGGCAGCCCTCTACTTTGGTGCTGCCCCGCCGCCTTCGCCCCCAAATAGCCAACTGGATTCCACTCCTCGCAGTGGCCAGGCCTTTCCGGGACTACCTCCCTACATGCTGCCCATGTCGCTGCCCATGGAGGCGCTGTTTAAGATGCGCCCGGGCGGAGACTTCGCTTCCAACCATGCCTTGATGAACAGTATTAAGCTGCCCGATTACCGGGGCACCAGCTTGAGTCCCGGTGGATCGGAGAAGCGTTCGTGGCGGGACGACGACTCGCGCATCTCACACGAGGACGAGTTCGGCGCCGGCGTTCTGATGCCACCGAAACCGCGTAGGGGCAAGGCGGAGACCCATGGACACGCTGGCGATCCCGATCTGCCCTATGTGTGCGATCAGTGCGACAAGGCCTTCGCCAAGCAGAGTTCGCTGGCGCGACACAAATACGAGCATTCCG
Seq C2 exon
GTCAACGACCCTACCAGTGCATAGAGTGTCCGAAGGCCTTCAAGCACAAGCACCACCTCACGGAGCACAAGCGTCTGCACAGCGGCGAGAAGCCCTTTCAGTGCTCCAAGTGCCTGAAGCGCTTCTCGCACTCGGGCAGCTACAGCCAGCACATGAACCACCGGTATTCCTATTGCAAGCCCTACAGGGAATAGGTAAGCGACACCTCAGTGCCGCCCACCTGCCAACCAGGAGCCAATCCCCTAGCGCCGCCAGCGATGGCCAGCAATCGGGGCCGTCGTCGAGTTCAGGTTCCGGCGGGGAATCCCCCGTACACGCCGCATCATCAGCATCCCCACCATCCGCATCTGGCGGCCATGGCTGCCTTCCAGCAGCAGCACCAGCAGCAGCAGCAACAGCATCAGCATCACAGGCTCTCCTGGAATGGAGGTGGAGTGATGCTGCCGCCGCCACCCAGTCAGCAGGCGCATCGTCCGACGGCTCCACATCCGCCTCCATTG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0004606-'4-5,'4-4,9-5=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.878 A=0.687 C2=0.556
Domain overlap (PFAM):
C1:
PF139121=zf-C2H2_6=PD(40.0=4.2),PF138941=zf-C2H2_4=PU(0.1=0.0)
A:
PF138941=zf-C2H2_4=PD(91.7=3.3),PF134651=zf-H2C2_2=WD(100=3.9),PF138941=zf-C2H2_4=WD(100=3.6),PF0004624=Homeobox=WD(100=8.5),PF134651=zf-H2C2_2=PU(38.5=1.5)
C2:
PF134651=zf-H2C2_2=PD(57.7=23.1),PF134651=zf-H2C2_2=WD(100=40.0)
Main Inclusion Isoform:
FBpp0303607
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
FBpp0085063, FBpp0085064
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGCAACTCCGCCTACGATTTG
R:
GTTCATGTGCTGGCTGTAGCT
Band lengths:
350-2349
Functional annotations
There are 1 annotated functions for this event
PMID: 10359772
This event
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: . ELM ID: ELMI000084; ELM sequence: PLDLS; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)