DmeINT0022093 @ dm6
Intron Retention
Gene
FBgn0001308 | Khc
Description
The gene Kinesin heavy chain is referred to in FlyBase by the symbol DmelKhc (CG7765, FBgn0001308). It is a protein_coding_gene from Dmel. It has 2 annotated transcripts and 2 polypeptides (1 unique). Gene sequence location is 2R:16266960..16271971. Its molecular function is described by 7 unique terms, many of which group under: hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides; hydrolase activity; pyrophosphatase activity; catalytic activity; nucleoside-triphosphatase activity. It is involved in the biological process described with 30 unique terms, many of which group under: microtubule sliding; protein-containing complex disassembly; cell cycle; visual system development; regulation of macromolecule metabolic process. 126 alleles are reported. The phenotypes of these alleles manifest in: intracellular; female reproductive system; cell projection; actin filament; female-specific anatomical entity. The phenotypic classes of alleles include: phenotype; increased mortality during development; behavior defective; visible. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed at stages throughout embryogenesis, during early larval stages, at stages throughout the pupal period, in adult female stages.
Coordinates
chr2R:16267776-16270253:-
Coord C1 exon
chr2R:16269794-16270253
Coord A exon
chr2R:16269656-16269793
Coord C2 exon
chr2R:16267776-16269655
Length
138 bp
Sequences
Splice sites
5' ss Seq
CAAGTGAGT
5' ss Score
9.1
3' ss Seq
CGATTTATCCATCCTTTCAGACA
3' ss Score
8.33
Exon sequences
Seq C1 exon
GGCAAGGTGTATTTGTTCGACAAGGTCTTCAAACCGAATGCATCCCAGGAGAAGGTCTACAATGAGGCGGCCAAGTCCATTGTTACGGATGTCCTGGCCGGGTACAATGGAACGATATTCGCATATGGTCAGACGTCCTCCGGAAAAACGCATACGATGGAGGGCGTGATCGGGGACTCCGTAAAACAGGGTATCATACCACGTATCGTCAACGACATTTTCAATCACATCTACGCGATGGAGGTGAACCTGGAGTTTCACATCAAGGTCTCCTACTACGAGATCTACATGGACAAGATTCGAGATCTGTTGGACGTCTCCAAGGTGAACTTAAGCGTGCACGAGGACAAGAACCGGGTGCCGTACGTCAAGGGCGCTACGGAACGGTTCGTCTCGTCGCCGGAGGATGTTTTCGAGGTGATCGAGGAGGGCAAATCCAATCGTCACATCGCTGTGACAA
Seq A exon
GTGAGTACTAATTAAAATCGATGGTCAGGACCATTTCAGCCAAACAAGTCTAGGAGTTATTCCAGCATTCGCTCTGACCTCGATTGTAGTGTTTCATGTACCAGATCATCTTTTATAACGATTTATCCATCCTTTCAG
Seq C2 exon
ACATGAACGAGCATTCTTCGCGATCCCACTCAGTATTCCTTATCAATGTGAAGCAGGAGAACCTGGAGAACCAGAAGAAACTATCCGGCAAACTCTACCTGGTGGATTTGGCCGGTTCCGAGAAGGTTTCCAAGACTGGAGCGGAGGGAACCGTTCTTGATGAAGCCAAGAACATCAACAAGTCGCTGTCGGCCTTGGGCAACGTAATTTCAGCCCTGGCGGACGGAAACAAAACGCACATCCCCTACCGTGATTCCAAGCTCACGCGCATCCTGCAGGAGTCGCTGGGAGGCAATGCACGCACAACCATCGTCATCTGCTGCTCTCCAGCCAGTTTCAACGAATCTGAAACGAAGTCAACGCTGGACTTCGGTCGTAGAGCCAAGACAGTGAAGAACGTGGTCTGCGTTAACGAGGAGCTTACTGCCGAGGAATGGAAGCGACGCTATGAAAAGGAGAAGGAAAAGAACGCCCGACTAAAGGGTAAGGTGGAGAAGCTGGAGATCGAGCTTGCGCGCTGGAGAGCGGGTGAAACTGTTAAGGCGGAGGAGCAAATCAACATGGAGGATCTCATGGAGGCAAGCACGCCCAACCTGGAAGTGGAGGCAGCACAGACGGCGGCGGCCGAGGCCGCTTTGGCCGCCCAGCGAACGGCTCTCGCCAATATGTCCGCATCGGTTGCCGTGAACGAGCAGGCCAGGCTGGCTACAGAGTGCGAGCGTCTCTACCAGCAGCTGGACGACAAGGATGAGGAGATCAATCAGCAGAGCCAGTACGCCGAGCAGCTCAAGGAGCAGGTGATGGAGCAGGAGGAACTCATCGCTAACGCTCGGCGTGAGTATGAGACTTTGCAGTCGGAGATGGCGCGAATCCAACAGGAGAACGAGTCCGCCAAGGAAGAGGTTAAGGAGGTGCTCCAAGCTCTCGAAGAGCTGGCTGTAAACTACGACCAGAAATCCCAGGAGATCGATAACAAGAACAAGGATATCGATGCCCTCAACGAGGAGCTGCAGCAGAAGCAGTCTGTGTTCAACGCCGCCTCCACAGAGCTACAGCAGCTCAAGGACATGTCCTCACACCAGAAGAAGCGCATCACGGAAATGCTAACCAACCTACTGCGCGACCTTGGCGAAGTGGGCCAGGCCATTGCCCCCGGCGAGTCCAGCATTGACCTCAAGATGAGTGCTCTGGCTGGCACGGATGCCAGCAAGGTGGAGGAAGATTTCACCATGGCGCGTTTGTTTATCAGCAAGATGAAGACGGAGGCCAAGAACATTGCCCAGCGATGCTCCAACATGGAAACACAGCAGGCTGACTCCAACAAGAAGATCTCCGAATATGAGAAAGATCTGGGCGAGTACCGGCTACTCATTTCGCAGCACGAGGCACGCATGAAGTCGCTGCAGGAGTCGATGCGGGAGGCAGAGAACAAGAAGCGCACGCTCGAGGAACAAATCGATTCGCTGCGCGAGGAATGCGCCAAGCTCAAGGCCGCCGAGCACGTTTCCGCCGTTAACGCCGAGGAGAAACAGCGGGCTGAGGAGCTGCGCTCCATGTTCGATTCTCAGATGGACGAGCTACGCGAAGCCCACACCCGGCAGGTGTCCGAGCTCCGGGATGAGATTGCCGCCAAGCAGCACGAAATGGACGAGATGAAGGATGTCCATCAAAAGCTGCTCTTGGCGCACCAACAGATGACGGCCGACTACGAGAAGGTGCGCCAGGAGGATGCCGAGAAGTCCAGCGAGCTTCAGAACATCATCCTCACCAACGAGCGTCGGGAGCAAGCGCGCAAAGACCTCAAGGGCCTGGAGGACACGGTGGCCAAGGAGTTGCAGACGCTACACAACCTGCGAAAACTTTTCGTTCAGGATCTACAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0001308:FBtr0087184:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.052 A=NA C2=0.450
Domain overlap (PFAM):
C1:
PF0022518=Kinesin=FE(48.4=100)
A:
NA
C2:
PF0022518=Kinesin=PD(41.1=20.7),PF045827=Reo_sigmaC=WD(100=18.7)
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0086328
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGACGTCTCCAAGGTGAACT
R:
ACCAGGTAGAGTTTGCCGGAT
Band lengths:
253-391
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)