DmeINT0028305 @ dm6
Intron Retention
Gene
FBgn0023479 | Tequila
Description
The gene Tequila is referred to in FlyBase by the symbol Dmel eq (CG4821, FBgn0023479). It is a protein_coding_gene from Dmel. It has 5 annotated transcripts and 5 polypeptides (4 unique). Gene sequence location is 3L:9074643..9092131. Its molecular function is described by: serine-type endopeptidase activity; chitin binding; scavenger receptor activity. It is involved in the biological process described with 6 unique terms, many of which group under: organic substance metabolic process; macromolecule metabolic process; nitrogen compound metabolic process; multicellular organismal process; system process. 13 alleles are reported. No phenotypic data is available. The phenotypic classes of alleles include: phenotype; increased mortality; stress response defective; visible. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of low expression to a trough of extremely low expression. Peak expression observed within 12-24 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes.
Coordinates
chr3L:9076319-9077456:+
Coord C1 exon
chr3L:9076319-9076795
Coord A exon
chr3L:9076796-9076857
Coord C2 exon
chr3L:9076858-9077456
Length
62 bp
Sequences
Splice sites
5' ss Seq
CCAGTAAGC
5' ss Score
5.34
3' ss Seq
GACCACTTTTTCGTTTACAGCTG
3' ss Score
10.28
Exon sequences
Seq C1 exon
CACTTATCCTTAATGCCTGTCCTGGTAACATCAACGGCATCTTCTTGTATCCCTACGATGCTAAAAAGTACGTTCAGTGCTCGTCTGGTGGCAGAATGTCCATCCTAAGTTGTGGCCCCCAGATGGCATTCAGTGTATCCCAAAGAAGTTGTCTTCCAAGTCATCAAGTGCACATATCTGATCGGGTGCAGTTCTGGCAGGAAGTCCAAGTTCAGACCACTTATACTTCTCAAGACTTACGGGGAAATGTTCAAAGCCAGCTGTCTTCACTTAGATCGTGTCCACCCAATGTCCAGAAGAATTACCCTTATCCATTCCATGCTGGCCACTATGTCAGGTGCCAATATGGAGCATTAGAGATCATATGCTGTCCCACTGGACAGCTTTACAGCCTCTCCCAGCGTCAATGTGTGCCTCGTAGCCTTCTCTCTGCACATGATTACTTGGATTACTCCTATATCAGTGCTGAACTTTCCA
Seq A exon
GTAAGCATCTCAAGGAATCTTGATGACTTGGACTTGATTTCAGACCACTTTTTCGTTTACAG
Seq C2 exon
CTGAGTTTATGGTGGATCGTTCGACGCTTTCTTGTCCTCCACAAGCACAGGGACTCTATCTGCATCCCTTCGATTGCACCAAGTATGTCAGGTGCTGGAATCAGCAGACCTTCATTGAGAGTTGTGTAGTATGTGCCTATGCAATATTAAGAACAATTAAATAAAATAGCATATTAACTTATGGCAGCACTTTGTTGCTATGTTTATGTTTATGTTTATGCACGCAGTTAGGCCAGGGCGGATGTAACATGATCACCCACTCGAAGGCCACAAAGTATAAGTGCATTGCCCACTCGAAGGCAAAAAGTATAAGTGCATGGTCAGCATTCACACGCCGACCAAATACATATTACATACGTACATACATATCTCGCTCTCCCGATAAGCCTAGATATATAAGATATACATAAGAACGCCGCTCCGCTGCTGGCGTACCCGGCAGCGCAGCTACGCGGATTAGCCTAAGTCCAAATATATAAAAAACTGTAAAATCGGAGAGACTCTGTAGACGTTGAGCTGACAGAACCATTTCTGCCTACTCTAAAATCAAAAGAAGAAATTGAATAAATATATGTCAGCCCGACGGCTGCCTTCAACTT
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0023479:FBtr0076527:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.011
Domain overlap (PFAM):
C1:
PF0160719=CBM_14=WD(100=33.1),PF0160719=CBM_14=WD(100=31.9)
A:
NA
C2:
PF0160719=CBM_14=PU(76.3=70.7)
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0311236
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
FBpp0076254, FBpp0311235
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCGTAGCCTTCTCTCTGCACA
R:
GTGCAATCGAAGGGATGCAGA
Band lengths:
141-203
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)