Special

DmeINT0031065 @ dm6

Intron Retention

Gene
FBgn0261397 | didum
Description
The gene dilute class unconventional myosin is referred to in FlyBase by the symbol Dmeldidum (CG2146, FBgn0261397). It is a protein_coding_gene from Dmel. It has 4 annotated transcripts and 4 polypeptides (3 unique). Gene sequence location is 2R:7500147..7508625. Its molecular function is described by 8 unique terms, many of which group under: binding; protein binding; cytoskeletal protein binding; nucleoside-triphosphatase activity; catalytic activity. It is involved in the biological process described with 9 unique terms, many of which group under: organelle organization; establishment of protein localization; positive regulation of multicellular organismal process; cellular component organization or biogenesis; actin filament-based process. 24 alleles are reported. The phenotypes of these alleles manifest in: reproductive system; adult abdomen; male organism; plasma membrane bounded cell projection; peripheral nervous system. The phenotypic classes of alleles include: lethal - all die before end of embryonic stage; increased mortality; fertile; phenotype. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, at stages throughout the pupal period, in stages of adults of both sexes.
Coordinates
chr2R:7502644-7503028:+
Coord C1 exon
chr2R:7502644-7502737
Coord A exon
chr2R:7502738-7502801
Coord C2 exon
chr2R:7502802-7503028
Length
64 bp
Sequences
Splice sites
5' ss Seq
CCAGTAAGT
5' ss Score
9.09
3' ss Seq
CTGTTAATTCTATCTTTGAGGCT
3' ss Score
3.69
Exon sequences
Seq C1 exon
ATGCCAAAGGGCTCGGACGAGAGCTGGGCTGGCAAGCTCATCGGAAAGTGCAATAAATTTCCGCATTTCGAGAAGCCACGCTTTGGCACAACCA
Seq A exon
GTAAGTTATTTTCCGATTCTATGCACTATTATTTCGATGACAATCTGTTAATTCTATCTTTGAG
Seq C2 exon
GCTTCTTTATCAAACATTTCTCGGACACGGTCGAGTATGACGTGAACGGATTCTTGGAAAAGAATCGTGACACAGTCTCCAAGGAGTTGACGCAAGTGCTAAGCGAGTCCAACATGTCTCTGGCCAAGCAGGTGATGACCCTGGAGGAAATAGATACTCTGTGCGTGGATTCCGCTAAATCCTCCACCTTGGGCGGCCGCGTCGTGATCAGTGCTGGCCGCAAACAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0261397:FBtr0089745:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact

ORF disruption upon sequence inclusion

No structure available
Features
Disorder rate (Iupred):
  C1=0.000 A=NA C2=0.022
Domain overlap (PFAM):

C1:
PF0006316=Myosin_head=FE(4.5=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(10.8=100)


Main Inclusion Isoform:
NA


Main Skipping Isoform:
FBpp0088684


Other Inclusion Isoforms:
NA


Other Skipping Isoforms:
FBpp0088685, FBpp0088686
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTTCCGCATTTCGAGAAGCCA
R:
CACTTGCGTCAACTCCTTGGA
Band lengths:
135-199
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Neural diversity
  • Neurogenesis
  • Neuronal activity
  • Splicing factor regulation (brain)
  • Splicing factor regulation (SL2)