DmeINT0033509 @ dm6
Intron Retention
Gene
FBgn0032252 | loh
Description
The gene lonely heart is referred to in FlyBase by the symbol Dmelloh (CG6232, FBgn0032252). It is a protein_coding_gene from Dmel. It has 4 annotated transcripts and 4 polypeptides (3 unique). Gene sequence location is 2L:10442534..10450080. Its molecular function is described by: peptidase activity. It is involved in the biological process described with: positive regulation of extracellular matrix assembly; larval heart development. 25 alleles are reported. The phenotypes of these alleles manifest in: embryonic/larval heart; embryonic/larval cardial cell; embryonic/larval pericardial cell; embryonic/larval alary muscle. The phenotypic classes of alleles include: lethal - all die during P-stage; viable; lethal - all die before end of larval stage; short lived; cell shape defective. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed within 12-24 hour embryonic stages.
Coordinates
chr2L:10444752-10445236:+
Coord C1 exon
chr2L:10444752-10444785
Coord A exon
chr2L:10444786-10445160
Coord C2 exon
chr2L:10445161-10445236
Length
375 bp
Sequences
Splice sites
5' ss Seq
CAGGTAAGA
5' ss Score
10.77
3' ss Seq
TTATTTAATTCTGTTTACAGTAT
3' ss Score
7.2
Exon sequences
Seq C1 exon
GCTTAGAAATGGCGAAGCTGTTGTTAATATTCAG
Seq A exon
GTAAGAAAATAGTATCTCAAACCGATGTGTAGCTCAACCTAAAAAAAAAAACGAATTCATGGATTTTTCAATAGATACAAATTTGATAGCACAAAAAGTGGGCGGGGTGAACATCTTGCTCAGCTGGATTTAATCAAGATTTGTTTGCCCTGCCGCCGAACAGTGGCTCGTTTTCACAGAAAACGTTGTAAAAAGTGTAGATCTAGATACTTCAAAGAAGTTTTTTTGCCTTTAAGATTGCATTGCTGTGTTCGGAATCAATCGGAAGTGATTCAGAGATGAGATGGAACTGATGAAACATTCAATTTCCGCTCTTAAAATATTCTCCTTTGCACACAAATATTGTATTTTTATTTTATTTAATTCTGTTTACAG
Seq C2 exon
TATTCTTACAATTCTCGCAACGATACACGATTCTAGTTTTGTGCAGGCGGATTTAACAACTAAAGAGCGAATTAAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0032252:FBtr0080029:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0079619
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
FBpp0307931
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
No suggested primer sequences
R:
No suggested primer sequences
Band lengths:
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)