DmeINT0033818 @ dm6
Intron Retention
Gene
FBgn0262732 | mbf1
Description
The gene multiprotein bridging factor 1 is referred to in FlyBase by the symbol Dmelmbf1 (CG4143, FBgn0262732). It is a protein_coding_gene from Dmel. It has 6 annotated transcripts and 6 polypeptides (2 unique). Gene sequence location is 3L:16574436..16576871. Its molecular function is described by: protein-macromolecule adaptor activity; transcription coactivator activity; sequence-specific DNA binding; mRNA binding. It is involved in the biological process described with: positive regulation of transcription by RNA polymerase II; central nervous system development; mRNA stabilization; open tracheal system development. 14 alleles are reported. The phenotypes of these alleles manifest in: mesothoracic tergum; tormogen cell; presumptive embryonic/larval tracheal system; trichogen cell; presumptive embryonic/larval central nervous system. The phenotypic classes of alleles include: increased mortality; phenotype; increased mortality during development; visible.
Coordinates
chr3L:16574645-16574976:+
Coord C1 exon
chr3L:16574645-16574752
Coord A exon
chr3L:16574753-16574852
Coord C2 exon
chr3L:16574853-16574976
Length
100 bp
Sequences
Splice sites
5' ss Seq
AATGTGAGT
5' ss Score
7.8
3' ss Seq
ACTATAAATCGACGAAAAAGATG
3' ss Score
-3.84
Exon sequences
Seq C1 exon
AGGCCTATTGCCGTTTAACCATCTGGTAACATTGAATTGAAGATAGTTCTGGAATTTCGTAGTTTTCATTAATAATAAAAGCAGATTTAGCAAAGCAAAGCAGCCAAT
Seq A exon
GTGAGTTCATTCGAACGCAGCGAGTCAACGAGGCGGTTAGTTCGCAAGTTTTCACTCATAAATTTTCATTTTTCGTCTTCACTATAAATCGACGAAAAAG
Seq C2 exon
ATGTCGGACTGGGACTCTGTGACTGTTCTTCGGAAGAAAGCACCGAAATCGTCGACCCTCAAGACGGAATCGGCGGTCAACCAGGCACGCCGTCAAGGAGTCGCCGTGGATACCCAACAGAAGT
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0262732:FBtr0075393:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
5' UTR
No structure available
Features
Disorder rate (Iupred):
C1=0.905 A=NA C2=0.905
Domain overlap (PFAM):
C1:
PF085235=MBF1=PU(57.1=95.2)
A:
NA
C2:
PF085235=MBF1=PU(57.1=95.2)
Main Inclusion Isoform:
FBpp0075152
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
FBpp0075151, FBpp0294020, FBpp0294021, FBpp0303170
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCCTATTGCCGTTTAACCATCTG
R:
CTTCTGTTGGGTATCCACGGC
Band lengths:
229-329
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)