DmeINT0033819 @ dm6
Intron Retention
Gene
FBgn0262732 | mbf1
Description
The gene multiprotein bridging factor 1 is referred to in FlyBase by the symbol Dmelmbf1 (CG4143, FBgn0262732). It is a protein_coding_gene from Dmel. It has 6 annotated transcripts and 6 polypeptides (2 unique). Gene sequence location is 3L:16574436..16576871. Its molecular function is described by: protein-macromolecule adaptor activity; transcription coactivator activity; sequence-specific DNA binding; mRNA binding. It is involved in the biological process described with: positive regulation of transcription by RNA polymerase II; central nervous system development; mRNA stabilization; open tracheal system development. 14 alleles are reported. The phenotypes of these alleles manifest in: mesothoracic tergum; tormogen cell; presumptive embryonic/larval tracheal system; trichogen cell; presumptive embryonic/larval central nervous system. The phenotypic classes of alleles include: increased mortality; phenotype; increased mortality during development; visible.
Coordinates
chr3L:16574853-16575347:+
Coord C1 exon
chr3L:16574853-16574976
Coord A exon
chr3L:16574977-16575186
Coord C2 exon
chr3L:16575187-16575347
Length
210 bp
Sequences
Splice sites
5' ss Seq
AGTGTGAGT
5' ss Score
6.96
3' ss Seq
TTTTAACCTTTTACGTACAGATG
3' ss Score
6.39
Exon sequences
Seq C1 exon
ATGTCGGACTGGGACTCTGTGACTGTTCTTCGGAAGAAAGCACCGAAATCGTCGACCCTCAAGACGGAATCGGCGGTCAACCAGGCACGCCGTCAAGGAGTCGCCGTGGATACCCAACAGAAGT
Seq A exon
GTGAGTGCCGGCCAAAAATCTCGTCCTCCTTCATTGATGAGTGACCCCCTTTTGCGACCACACCACTGTCGTCTAACCTAGAATCGTCTCCTGCTGCATTGCGTGGCAATTCACTTGCTGCCGCCAATTTCACAATCGCAAGCAAACCTTTCACGCTGCCCGTGTGAACTCCCCTCTAGTAATGACACACTTTTAACCTTTTACGTACAG
Seq C2 exon
ATGGTGCTGGCACCAACAAGCAGCATGTGACCACCAAGAACACTGCCAAACTAGACCGCGAGACCGAGGAGCTGCGCCACGACAAGATTCCCCTCGATGTGGGCAAGCTCATCCAGCAGGGACGACAGAGCAAGGGCCTCAGCCAAAAGGACCTGGCAACT
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0262732:FBtr0075393:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.905 A=NA C2=0.919
Domain overlap (PFAM):
C1:
PF085235=MBF1=PU(57.1=95.2)
A:
NA
C2:
PF085235=MBF1=PD(41.4=53.7),PF0138117=HTH_3=PU(29.6=29.6)
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0303170
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
FBpp0075151, FBpp0075152, FBpp0294020, FBpp0294021
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGTCGGACTGGGACTCTGTG
R:
AGTTGCCAGGTCCTTTTGG
Band lengths:
285-495
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)