DmeINT0037971 @ dm6
Intron Retention
Gene
FBgn0034013 | unc-5
Description
The gene unc-5 is referred to in FlyBase by the symbol Dmelunc-5 (CG8166, FBgn0034013). It is a protein_coding_gene from Dmel. It has 3 annotated transcripts and 3 polypeptides (1 unique). Gene sequence location is 2R:15340472..15368300. Its molecular function is described by: netrin receptor activity involved in chemorepulsion; netrin receptor activity. It is involved in the biological process described with 6 unique terms, many of which group under: multicellular organism development; anatomical structure morphogenesis; developmental process; regulation of locomotion; plasma membrane bounded cell projection morphogenesis. 29 alleles are reported. The phenotypes of these alleles manifest in: plasma membrane bounded cell projection; cell projection; dorsal vessel primordium; visual system neuron; filopodium. The phenotypic classes of alleles include: increased mortality; cell migration defective; phenotype; increased mortality during development. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-12 hour embryonic stages.
Coordinates
chr2R:15358009-15358749:+
Coord C1 exon
chr2R:15358009-15358290
Coord A exon
chr2R:15358291-15358578
Coord C2 exon
chr2R:15358579-15358749
Length
288 bp
Sequences
Splice sites
5' ss Seq
ATGGTCAGT
5' ss Score
6.57
3' ss Seq
TTTCCGTTTTCATCCCACAGTCA
3' ss Score
10.4
Exon sequences
Seq C1 exon
ATATTCGCAAGTCCTTCAACCAGTCGCCCACCTCGCTGCGCCTGGAGCTGGGCAGTCGGGCGGAACTGCGCTGCGAACCACCCGGCGGTTTCCCCGAACCGAAGCTCACCTGGCACAAAAACAACGCGGTCATCACGGCGGACAGCGAGCCGGGGATCACCGTTTCGGCCGGCACACTCATCTTCCGCCAGGTGGCCCTGCAGCATATGGCCAACTATAGCTGCAGTGCGGAGAATATCGCTGGCAGACGCGTCTCCGATTCCGCCGTGCTCATCGTTTATG
Seq A exon
GTCAGTACAGATGGGCTTTGGGGCAAATTAAAAATTTATAGGTTTCATGCTGTAAATTCCAGAAAGAAACCCCATTTACTGGGGGTTCTCGGTTTTAATCATTTTCCATGAAATTTTATTAAATTTATTGCTAATAGAACTGAGGAACTATAATCATTTTACACTAATATACTCTTAAATTATATGGCCAAAAATCGCATAGCACCTTAAATGGAGTAGCGAAAAATCATTTGGTATTGCAGGTCGATAAAGCTCTTAATTGTTTTATTTTCCGTTTTCATCCCACAG
Seq C2 exon
TCAACGGTGGCTGGAGCACCTGGAGTCCATGGCGCGAATGCAAGTGTGCGGGCAAGCCCAGCCAGGGAAGGAAGCGTTCGCGCACCTGCAACAATCCGATGCCATTGAATGGGGGCGCCCAGTGTCCGGGCCCCCAGATCCAGAAGTCCGCCGACTGCGCCGCATGTCCAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0034013:FBtr0087382:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.053 A=NA C2=0.172
Domain overlap (PFAM):
C1:
PF0767911=I-set=WD(100=94.7)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Main Skipping Isoform:
FBpp0086514

Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
FBpp0309017
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCGCAAGTCCTTCAACCAGTC
R:
ATGGACTCCAGGTGCTCCAG
Band lengths:
308-596
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)